Band-like differentiation in mitotic prometaphase chromosomes of celery

Minoru Murata; Thomas J. Orton

doi:10.1093/oxfordjournals.jhered.a109917

Band-like differentiation in mitotic prometaphase chromosomes of celery

Minoru Murata
, Thomas J. Orton

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Mitotic prometaphase chromosomes of celery (Apium graveolens) stained with acetocarmine exhibited a large number of interstitial heterochromatic bands that could not be resolved at metaphase. Comparison between these banding patterns and those produced by Giemsa C-banding showed that 1) interstitial bands were not stained by Giemsa, 2) C-bands appeared at all centromere regions, and 3) telomeric regions were variable in affinity to acetocarmine and Giemsa. These results strongly suggest that the differentiation in prometaphase chromosomes of celery is equivalent to G-bands obtained in metaphase chromosomes by higher vertebrates.

Original language	English (US)
Pages (from-to)	225-228
Number of pages	4
Journal	Journal of Heredity
Volume	75
Issue number	3
DOIs	https://doi.org/10.1093/oxfordjournals.jhered.a109917
State	Published - May 1984
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Molecular Biology
Genetics
Genetics(clinical)

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10.1093/oxfordjournals.jhered.a109917

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@article{c013a077f9924c4a97cfe89402767773,

title = "Band-like differentiation in mitotic prometaphase chromosomes of celery",

abstract = "Mitotic prometaphase chromosomes of celery (Apium graveolens) stained with acetocarmine exhibited a large number of interstitial heterochromatic bands that could not be resolved at metaphase. Comparison between these banding patterns and those produced by Giemsa C-banding showed that 1) interstitial bands were not stained by Giemsa, 2) C-bands appeared at all centromere regions, and 3) telomeric regions were variable in affinity to acetocarmine and Giemsa. These results strongly suggest that the differentiation in prometaphase chromosomes of celery is equivalent to G-bands obtained in metaphase chromosomes by higher vertebrates.",

author = "Minoru Murata and Orton, \{Thomas J.\}",

note = "Funding Information: The authors are, respectively, former postdoctoral fellow, and former assistant professor, Department of Vegetable Crops, University of California, Davis, CA 95616. Their present addresses are: College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252, Japan; and Agrigenetics, Inc., 3375 Mitchell Lane, Boulder, Colorado 80301. This work wassupported by USDA/ CRGO grant 59-2063-01449. Please address reprint requests to Dr. Murata. {\textcopyright} 1984, American Genetic Association.",

year = "1984",

month = may,

doi = "10.1093/oxfordjournals.jhered.a109917",

language = "English (US)",

volume = "75",

pages = "225--228",

journal = "Journal of Heredity",

issn = "0022-1503",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Band-like differentiation in mitotic prometaphase chromosomes of celery

AU - Murata, Minoru

AU - Orton, Thomas J.

N1 - Funding Information: The authors are, respectively, former postdoctoral fellow, and former assistant professor, Department of Vegetable Crops, University of California, Davis, CA 95616. Their present addresses are: College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252, Japan; and Agrigenetics, Inc., 3375 Mitchell Lane, Boulder, Colorado 80301. This work wassupported by USDA/ CRGO grant 59-2063-01449. Please address reprint requests to Dr. Murata. © 1984, American Genetic Association.

PY - 1984/5

Y1 - 1984/5

N2 - Mitotic prometaphase chromosomes of celery (Apium graveolens) stained with acetocarmine exhibited a large number of interstitial heterochromatic bands that could not be resolved at metaphase. Comparison between these banding patterns and those produced by Giemsa C-banding showed that 1) interstitial bands were not stained by Giemsa, 2) C-bands appeared at all centromere regions, and 3) telomeric regions were variable in affinity to acetocarmine and Giemsa. These results strongly suggest that the differentiation in prometaphase chromosomes of celery is equivalent to G-bands obtained in metaphase chromosomes by higher vertebrates.

AB - Mitotic prometaphase chromosomes of celery (Apium graveolens) stained with acetocarmine exhibited a large number of interstitial heterochromatic bands that could not be resolved at metaphase. Comparison between these banding patterns and those produced by Giemsa C-banding showed that 1) interstitial bands were not stained by Giemsa, 2) C-bands appeared at all centromere regions, and 3) telomeric regions were variable in affinity to acetocarmine and Giemsa. These results strongly suggest that the differentiation in prometaphase chromosomes of celery is equivalent to G-bands obtained in metaphase chromosomes by higher vertebrates.

UR - https://www.scopus.com/pages/publications/0040734737

UR - https://www.scopus.com/pages/publications/0040734737#tab=citedBy

U2 - 10.1093/oxfordjournals.jhered.a109917

DO - 10.1093/oxfordjournals.jhered.a109917

M3 - Article

AN - SCOPUS:0040734737

SN - 0022-1503

VL - 75

SP - 225

EP - 228

JO - Journal of Heredity

JF - Journal of Heredity

IS - 3

ER -

Band-like differentiation in mitotic prometaphase chromosomes of celery

Abstract

All Science Journal Classification (ASJC) codes

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