Behaviour of fibroblasts and epidermal cells cultivated on analogues of extracellular matrix

Charles J. Doillon, Arthur J. Wasserman, Richard A. Berg, Frederick H. Silver

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


A porous collagen sponge can be used for supporting epidermal cells and fibroblasts in order to manufacture an artificial skin. Fibroblasts were grown on analogues of extracellular matrix containing collagen and glycosaminoglycans and/or glycoproteins. Cell replication, and also infiltration of fibroblasts, were enhanced by the presence of hyaluronic acid and/or fibronectin. Epidermal cells grown on a collagen sponge have been characterized by microscopic observations. Epidermal cells on the surface of the sponge showed an incomplete differentiation in comparison to normal skin; clumps of epidermal cells were found in the interior of the sponge. Epidermal cell replication was enhanced in the presence of collagen sponge seeded with fibroblasts.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
Issue number1
StatePublished - Jan 1988

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


  • Collagen
  • epidermal cells
  • extracellular matrix
  • fibroblasts
  • fibronectin
  • hyaluronic acid

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