Binding of the initiation factor σ70 to core RNA polymerase is a multistep process

Tanja M. Gruber, Dmitriy Markov, Meghan M. Sharp, Brian A. Young, Chi Zen Lu, Hong Ji Zhong, Irina Artsimovitch, Katherine M. Geszvain, Terrance M. Arthur, Richard R. Burgess, Robert Landick, Konstantin Severinov, Carol A. Gross

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and σ70, the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between σ70 and specific domains of RNA polymerase and provide evidence that σ70 and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of σ70 with core RNA polymerase is associated with progression through the process of transcription initiation.

Original languageEnglish (US)
Pages (from-to)21-31
Number of pages11
JournalMolecular cell
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2001

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Peptide Initiation Factors
DNA-Directed RNA Polymerases
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Gruber, T. M., Markov, D., Sharp, M. M., Young, B. A., Lu, C. Z., Zhong, H. J., ... Gross, C. A. (2001). Binding of the initiation factor σ70 to core RNA polymerase is a multistep process. Molecular cell, 8(1), 21-31. https://doi.org/10.1016/S1097-2765(01)00292-1
Gruber, Tanja M. ; Markov, Dmitriy ; Sharp, Meghan M. ; Young, Brian A. ; Lu, Chi Zen ; Zhong, Hong Ji ; Artsimovitch, Irina ; Geszvain, Katherine M. ; Arthur, Terrance M. ; Burgess, Richard R. ; Landick, Robert ; Severinov, Konstantin ; Gross, Carol A. / Binding of the initiation factor σ70 to core RNA polymerase is a multistep process. In: Molecular cell. 2001 ; Vol. 8, No. 1. pp. 21-31.
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Gruber, TM, Markov, D, Sharp, MM, Young, BA, Lu, CZ, Zhong, HJ, Artsimovitch, I, Geszvain, KM, Arthur, TM, Burgess, RR, Landick, R, Severinov, K & Gross, CA 2001, 'Binding of the initiation factor σ70 to core RNA polymerase is a multistep process', Molecular cell, vol. 8, no. 1, pp. 21-31. https://doi.org/10.1016/S1097-2765(01)00292-1

Binding of the initiation factor σ70 to core RNA polymerase is a multistep process. / Gruber, Tanja M.; Markov, Dmitriy; Sharp, Meghan M.; Young, Brian A.; Lu, Chi Zen; Zhong, Hong Ji; Artsimovitch, Irina; Geszvain, Katherine M.; Arthur, Terrance M.; Burgess, Richard R.; Landick, Robert; Severinov, Konstantin; Gross, Carol A.

In: Molecular cell, Vol. 8, No. 1, 01.01.2001, p. 21-31.

Research output: Contribution to journalArticle

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AU - Gruber, Tanja M.

AU - Markov, Dmitriy

AU - Sharp, Meghan M.

AU - Young, Brian A.

AU - Lu, Chi Zen

AU - Zhong, Hong Ji

AU - Artsimovitch, Irina

AU - Geszvain, Katherine M.

AU - Arthur, Terrance M.

AU - Burgess, Richard R.

AU - Landick, Robert

AU - Severinov, Konstantin

AU - Gross, Carol A.

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