Bioactive homoisoflavones from Vietnamese coriander or pak pai (Polygonatum odoratum)

Bret C. Vastano, M. Mohamed Rafi, Robert S. DiPaola, Nanqun Zhu, Chi Tang Ho, Anthony T. Rella, Geetha Ghai, Robert T. Rosen

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The roots of Polygonatum odoratum were screened for compounds which induce apoptosis in breast cancer cell lines. The powdered roots were extracted with methanol and ethyl acetate. The ethyl acetate fraction was then subjected to bioassay directed fractionation using silica gel column chromatography. Bcl-2 protein, a regulator of apoptosis, was assessed by immunoblot. The active fraction was eluted with 30:1 CHCl3/CH3OH and was rechromatographed on a second silica gel column. Elution with 20:80 hexanes/chloroform yielded the active fraction which was purified using semipreparative HPLC. Two compounds were identified. These include 2,3-dihydro-3-[(15-hydroxyphenyl)methyl]-5,7-dihydroxy-6-methyl-8-methoxy-4H-1- benzopyran-4-one and 2,3-dihydro-3-[(15-hydroxyphenyl)-methyl]-5,7-dihydroxy-6,8-dimethyl-4H-1- benzopyran-4-one.

Original languageEnglish (US)
Pages (from-to)269-280
Number of pages12
JournalACS Symposium Series
Volume803
StatePublished - Dec 1 2002

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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