Bioactive peptides/chitosan nanoparticles enhance cellular antioxidant activity of (-)-epigallocatechin-3-gallate

Bing Hu, Yuwen Ting, Xiaoxiong Zeng, Qingrong Huang

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

(-)-Epigallocatechin-3-gallate (EGCG), one representative of the well-studied chemopreventive phytochemicals but with low bioavailability, was encapsulated in monodispersed nanoparticles that were assembled from bioactive caseinophosphopeptide (CPP) and chitosan (CS). The encapsulation efficiency of EGCG in CS-CPP nanoparticles ranged from 70.5 to 81.7%; meanwhile, the in vitro release of EGCG from CS-CPP nanoparticles was in a controllable manner. The EGCG-loaded CS-CPP nanoparticles exerted stronger activity of scavenging free radical than the free EGCG (p < 0.01) in the cellular antioxidant activity assay. Furthermore, cellular uptake of the EGCG-loaded CS-CPP nanoparticles was confirmed by the green fluorescence inside the human hepatocellular caricinoma (HepG2) cells, which was considered to play an important role in the improvement of the antioxidant activity of the nanoencapsulated EGCG. The results suggested that encapsulation of EGCG using CS-CPP nanoparticles should be a potential approach to enhance its antioxidant activity in biological systems.

Original languageEnglish (US)
Pages (from-to)875-881
Number of pages7
JournalJournal of agricultural and food chemistry
Volume61
Issue number4
DOIs
StatePublished - Jan 30 2013

Fingerprint

epigallocatechin
Chitosan
nanoparticles
chitosan
Nanoparticles
Antioxidants
antioxidant activity
peptides
Peptides
encapsulation
Encapsulation
epigallocatechin gallate
Scavenging
Hep G2 Cells
Phytochemicals
Biological systems
phytopharmaceuticals
Biological Availability
Free Radicals
bioavailability

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • EGCG
  • caseinophosphopeptide
  • cellular antioxidant activity
  • cellular uptake
  • chitosan
  • nanoparticle

Cite this

@article{98776ec809e440929d7c6cf8a70bfd46,
title = "Bioactive peptides/chitosan nanoparticles enhance cellular antioxidant activity of (-)-epigallocatechin-3-gallate",
abstract = "(-)-Epigallocatechin-3-gallate (EGCG), one representative of the well-studied chemopreventive phytochemicals but with low bioavailability, was encapsulated in monodispersed nanoparticles that were assembled from bioactive caseinophosphopeptide (CPP) and chitosan (CS). The encapsulation efficiency of EGCG in CS-CPP nanoparticles ranged from 70.5 to 81.7{\%}; meanwhile, the in vitro release of EGCG from CS-CPP nanoparticles was in a controllable manner. The EGCG-loaded CS-CPP nanoparticles exerted stronger activity of scavenging free radical than the free EGCG (p < 0.01) in the cellular antioxidant activity assay. Furthermore, cellular uptake of the EGCG-loaded CS-CPP nanoparticles was confirmed by the green fluorescence inside the human hepatocellular caricinoma (HepG2) cells, which was considered to play an important role in the improvement of the antioxidant activity of the nanoencapsulated EGCG. The results suggested that encapsulation of EGCG using CS-CPP nanoparticles should be a potential approach to enhance its antioxidant activity in biological systems.",
keywords = "EGCG, caseinophosphopeptide, cellular antioxidant activity, cellular uptake, chitosan, nanoparticle",
author = "Bing Hu and Yuwen Ting and Xiaoxiong Zeng and Qingrong Huang",
year = "2013",
month = "1",
day = "30",
doi = "10.1021/jf304821k",
language = "English (US)",
volume = "61",
pages = "875--881",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "4",

}

Bioactive peptides/chitosan nanoparticles enhance cellular antioxidant activity of (-)-epigallocatechin-3-gallate. / Hu, Bing; Ting, Yuwen; Zeng, Xiaoxiong; Huang, Qingrong.

In: Journal of agricultural and food chemistry, Vol. 61, No. 4, 30.01.2013, p. 875-881.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioactive peptides/chitosan nanoparticles enhance cellular antioxidant activity of (-)-epigallocatechin-3-gallate

AU - Hu, Bing

AU - Ting, Yuwen

AU - Zeng, Xiaoxiong

AU - Huang, Qingrong

PY - 2013/1/30

Y1 - 2013/1/30

N2 - (-)-Epigallocatechin-3-gallate (EGCG), one representative of the well-studied chemopreventive phytochemicals but with low bioavailability, was encapsulated in monodispersed nanoparticles that were assembled from bioactive caseinophosphopeptide (CPP) and chitosan (CS). The encapsulation efficiency of EGCG in CS-CPP nanoparticles ranged from 70.5 to 81.7%; meanwhile, the in vitro release of EGCG from CS-CPP nanoparticles was in a controllable manner. The EGCG-loaded CS-CPP nanoparticles exerted stronger activity of scavenging free radical than the free EGCG (p < 0.01) in the cellular antioxidant activity assay. Furthermore, cellular uptake of the EGCG-loaded CS-CPP nanoparticles was confirmed by the green fluorescence inside the human hepatocellular caricinoma (HepG2) cells, which was considered to play an important role in the improvement of the antioxidant activity of the nanoencapsulated EGCG. The results suggested that encapsulation of EGCG using CS-CPP nanoparticles should be a potential approach to enhance its antioxidant activity in biological systems.

AB - (-)-Epigallocatechin-3-gallate (EGCG), one representative of the well-studied chemopreventive phytochemicals but with low bioavailability, was encapsulated in monodispersed nanoparticles that were assembled from bioactive caseinophosphopeptide (CPP) and chitosan (CS). The encapsulation efficiency of EGCG in CS-CPP nanoparticles ranged from 70.5 to 81.7%; meanwhile, the in vitro release of EGCG from CS-CPP nanoparticles was in a controllable manner. The EGCG-loaded CS-CPP nanoparticles exerted stronger activity of scavenging free radical than the free EGCG (p < 0.01) in the cellular antioxidant activity assay. Furthermore, cellular uptake of the EGCG-loaded CS-CPP nanoparticles was confirmed by the green fluorescence inside the human hepatocellular caricinoma (HepG2) cells, which was considered to play an important role in the improvement of the antioxidant activity of the nanoencapsulated EGCG. The results suggested that encapsulation of EGCG using CS-CPP nanoparticles should be a potential approach to enhance its antioxidant activity in biological systems.

KW - EGCG

KW - caseinophosphopeptide

KW - cellular antioxidant activity

KW - cellular uptake

KW - chitosan

KW - nanoparticle

UR - http://www.scopus.com/inward/record.url?scp=84873206534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873206534&partnerID=8YFLogxK

U2 - 10.1021/jf304821k

DO - 10.1021/jf304821k

M3 - Article

C2 - 23293838

AN - SCOPUS:84873206534

VL - 61

SP - 875

EP - 881

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 4

ER -