Biochemical analysis of cybrids expressing mitochondrial DNA from Contursi kindred Parkinson's subjects

Russell H. Swerdlow, Janice K. Parks, David S. Cassarino, Daniel R. Binder, James P. Bennett, Giuseppe Di Iorio, Lawrence I. Golbe, W. Davis Parker

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41 Scopus citations


Complex I activity is reduced in cytoplasmic hybrid (cybrid) cell lines that contain mitochondrial DNA (mtDNA) from sporadic Parkinson's disease (PD) patients. This implies that mtDNA aberration occurs in sporadic PD. To assess the integrity of mtDNA in autosomal dominant PD arising from mutation of the α-synuclein gene, we transferred mitochondrial genes from PD-affected members of the Italian-American Contursi kindred to cells previously depleted of their endogenous mtDNA. Unlike cybrid cell lines expressing mtDNA from persons with sporadic or maternally inherited PD, the resultant Contursi cybrid lines did not manifest complex I deficiency, indicating that in Contursi PD mtDNA integrity is relatively preserved. Compared to control cybrids, however, Contursi cyo brid lines did show some evidence of oxidative stress. For reasons that are unclear, at least a limited amount of mtDNA damage may nevertheless develop in PD patients with α-synuclein mutation.

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalExperimental Neurology
Issue number2
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience


  • Cybrids
  • Mitochondria
  • Mitochondrial DNA
  • Oxidative stress
  • Parkinson's disease
  • α-synuclein


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