Biochemistry and structural biology of transcription factor IID (TFIID)

S. K. Burley, R. G. Roeder

Research output: Contribution to journalReview articlepeer-review

633 Scopus citations


Eukaryotes have three distinct RNA polymerases that catalyze transcription of nuclear genes. RNA polymerase II is responsible for transcribing nuclear genes encoding the messenger RNAs and several small nuclear RNAs. Like RNA polymerases I and III, pol II cannot recognize its target promoter directly and initiate transcription without accessory factors. Instead, this large multisubunit enzyme relies on both general transcription factors and transcriptional activators and coactivators to regulate transcription from class II promoters. At the center of this process is TFIID, a 700-kD complex composed of the TATA box binding protein (TBP) and a set of phylogenetically conserved, polymerase-specific TBP-associated factors or TAF(II)s. Together, TBP and the TAF(II)s direct assembly of the transcription machinery and play critical regulatory roles in eukaryotic gene expression.

Original languageEnglish (US)
Pages (from-to)769-799
Number of pages31
JournalAnnual review of biochemistry
StatePublished - 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry


  • RNA polymerase II
  • TAF
  • TATA box binding protein
  • TBP
  • TBP-associated factors
  • eukaryotic transcription
  • histone proteins
  • transcriptional activators


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