Since 1982, Interregional Research Project Number 4 (IR-4) has assisted in the registration of biopesticides with the United States Environmental Protection Agency (EPA). An explanation is provided of some of the registrations recently obtained by IR-4 and how the regulations in EPA have changed. Additionlly, an update is included how new toxicology tests are being evaluated which are more animal friendly. Regulations regarding RNAi are still under development. Finally, how the fee for service system of EPA under the Pesticide Registration Improvement Act operates is described.