Biosynthesis of Aflatoxin B1. Conversion of Versicolorin A to Aflatoxin B1 by Aspergillus parasiticus

Louise S. Lee; Joan W. Bennett; Alva F. Cucullu; Robert L. Ory

doi:10.1021/jf60208a017

Biosynthesis of Aflatoxin B₁. Conversion of Versicolorin A to Aflatoxin B₁ by Aspergillus parasiticus

Louise S. Lee, Joan W. Bennett, Alva F. Cucullu, Robert L. Ory

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50 Scopus citations

Abstract

Forty-six percent of added [¹⁴C] versicolorin A was efficiently converted to aflatoxin B₁ by a resting cell culture of wild-type Aspergillus parasiticus. The labeled pigment was isolated from [1-¹⁴C]acetate enriched cultures of a mutant of A. parasiticus that elaborates several versicolorin pigments, but no aflatoxins. The high level of incorporation into aflatoxin B₁ and the relative specific activity of 0.475 of aflatoxin isolated from the culture of A. parasiticus enriched with [¹⁴C]versicolorin A indicate that this C-18 polyketide-derived hydroxyanthraquinone is a precursor to aflatoxin B₁.

Original language	English (US)
Pages (from-to)	1167-1170
Number of pages	4
Journal	Journal of agricultural and food chemistry
Volume	24
Issue number	6
DOIs	https://doi.org/10.1021/jf60208a017
State	Published - Nov 1 1976
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)
Agricultural and Biological Sciences(all)

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10.1021/jf60208a017

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title = "Biosynthesis of Aflatoxin B1. Conversion of Versicolorin A to Aflatoxin B1 by Aspergillus parasiticus",

abstract = "Forty-six percent of added [14C] versicolorin A was efficiently converted to aflatoxin B1 by a resting cell culture of wild-type Aspergillus parasiticus. The labeled pigment was isolated from [1-14C]acetate enriched cultures of a mutant of A. parasiticus that elaborates several versicolorin pigments, but no aflatoxins. The high level of incorporation into aflatoxin B1 and the relative specific activity of 0.475 of aflatoxin isolated from the culture of A. parasiticus enriched with [14C]versicolorin A indicate that this C-18 polyketide-derived hydroxyanthraquinone is a precursor to aflatoxin B1.",

author = "Lee, {Louise S.} and Bennett, {Joan W.} and Cucullu, {Alva F.} and Ory, {Robert L.}",

year = "1976",

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doi = "10.1021/jf60208a017",

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TY - JOUR

T1 - Biosynthesis of Aflatoxin B1. Conversion of Versicolorin A to Aflatoxin B1 by Aspergillus parasiticus

AU - Lee, Louise S.

AU - Bennett, Joan W.

AU - Cucullu, Alva F.

AU - Ory, Robert L.

PY - 1976/11/1

Y1 - 1976/11/1

N2 - Forty-six percent of added [14C] versicolorin A was efficiently converted to aflatoxin B1 by a resting cell culture of wild-type Aspergillus parasiticus. The labeled pigment was isolated from [1-14C]acetate enriched cultures of a mutant of A. parasiticus that elaborates several versicolorin pigments, but no aflatoxins. The high level of incorporation into aflatoxin B1 and the relative specific activity of 0.475 of aflatoxin isolated from the culture of A. parasiticus enriched with [14C]versicolorin A indicate that this C-18 polyketide-derived hydroxyanthraquinone is a precursor to aflatoxin B1.

AB - Forty-six percent of added [14C] versicolorin A was efficiently converted to aflatoxin B1 by a resting cell culture of wild-type Aspergillus parasiticus. The labeled pigment was isolated from [1-14C]acetate enriched cultures of a mutant of A. parasiticus that elaborates several versicolorin pigments, but no aflatoxins. The high level of incorporation into aflatoxin B1 and the relative specific activity of 0.475 of aflatoxin isolated from the culture of A. parasiticus enriched with [14C]versicolorin A indicate that this C-18 polyketide-derived hydroxyanthraquinone is a precursor to aflatoxin B1.

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JO - Journal of Agricultural and Food Chemistry

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Biosynthesis of Aflatoxin B₁. Conversion of Versicolorin A to Aflatoxin B₁ by Aspergillus parasiticus

Abstract

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