Blastocyst injection of embryonic stem cells: A simple approach to Unveil mechanisms of corrections in mouse models of human disease

Joel S. Schneider, Joseph M. Vitale, Andre Terzic, Diego Fraidenraich

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Embryonic stem cell (ESC) research is a promising area of investigation with enormous therapeutic potential. We have injected murine wild type (WT) ESCs into a variety of mutant murine blastocysts, which are predisposed to develop a human-like disease, such as muscular dystrophy or the embryonic lethal "thin myocardial syndrome". In this review, we summarize data indicating that partial incorporation of ESCs is sufficient to prevent disease from occurring. We also present data indicating that blastocyst incorporation of ESCs may aid in the prevention of heart failure in stressed WT mice. In some cases, the rescue observed is predominantly non-cell autonomous and relies on the production of secreted factors from the ES-derived cells, but in others, cell replacement is required. Thus, congenital or acquired disease can be pre-emptively averted in mice by developmental injection of ESCs.

Original languageEnglish (US)
Pages (from-to)369-377
Number of pages9
JournalStem Cell Reviews and Reports
Volume5
Issue number4
DOIs
StatePublished - Dec 1 2009

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Cancer Research

Keywords

  • Blastocyst
  • Congenital heart disease
  • Embryonic stem cells
  • Mouse model of human disease
  • Muscular dystrophy
  • Myocardial infarction

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