Blockade of bepridil on I(A) and I(K) in acutely hippocampal CA1 neurons

Huijuan Hu, Dongmei Yang, Zuojun Lin, Caihong Wu, Pei'Ai Zhou, Dezai Dai

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16 Scopus citations


The effects of bepridil, an antianginal agent with antiarrhythmic action, on voltage-dependent K+ currents in the CA1 pyramidal neurons acutely isolated from rat hippocampus were studied by means of whole-cell patch clamp techniques. Current recordings were made in the presence of TTX to block Na+ current. Depolarizing test pulses activated two components of outward K+ currents: a rapidly activating and inactivating component, I(A); and a delayed component, I(K). Results showed that bepridil reduced the amplitude of I(A) and I(K), and exerted its inhibitory action in time- and dose-dependent manner. Half-blocking concentrations (IC50) of bepridil on I(A) and I(K) were 17.8 μM and 1.7 μM, respectively. 10 μM bepridil suppressed I(A) and I(K) by 46.7% and 77.1% at +30 mV of depolarization, respectively. When I(K) was activated nearly uncontaminated with I(A) by holding at -50 mV, 10 μM bepridil inhibited I(K) by 71.6% at +30 mV of depolarization; 10 μM bepridil positively shifted the voltage-dependent of activation curves of I(A) and I(K) 12.1 mV and 28.7 mV, respectively. These results suggested that blockade on K+ currents by bepridil is preferential for I(K), and contributes to the protection brain against ischemic damage.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalBrain research
Issue number2
StatePublished - Nov 2 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


  • Bepridil
  • CA1 neuron
  • Hippocampus
  • Patch-clamp
  • Potassium current


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