Blockage of substance P-induced scratching behavior in rats by the intrathecal administration of inhibitory amino acid agonists

Carlos Beyer, Cynthia Banas, Oscar Gonzalez-Flores, Barry R. Komisaruk

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Intrathecal administration of 20 μg of substance P induced scratching behavior in most tested rats (80%). Scratching appeared in bouts of short latency and variable duration, intensity and frequency (range 1-60, mean number of scratching bouts in one hour test: 8.93±1.86). Intrathecal administration of glycine (400 μg but not 66 μg) significantly decreased the effect of substance P on this behavior. Taurine, in dosages equimolar to glycine, abolished the response to substance P at the high dose level (700 μg), but did not significantly affect it at the lower level (120 μg). The GABAA agonist, muscimol, abolished the effect of substance P at the 3 μg dose level, but the 0.5 μg dose did not produce a significant effect. Baclofen, a GABAB agonist, was highly effective in significantly reducing the action of SP at 0.9 and 0.15 μg; only two of 8 rats receiving the low dose of baclofen (0.15 μg) exhibited scratching. The results suggest that the spinal inhibitory amino acids modulate nociceptive impulses generated by the action of substance P in dorsal horn neurons of the spinothalamic tract.

Original languageEnglish (US)
Pages (from-to)491-495
Number of pages5
JournalPharmacology, Biochemistry and Behavior
Volume34
Issue number3
DOIs
StatePublished - Nov 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

Keywords

  • Baclofen
  • Glycine
  • Inhibitory amino acids
  • Intrathecal administration
  • Itch
  • Rats, female
  • Scratching
  • Substance P
  • Taurine

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