TY - JOUR
T1 - Boundaries defined by adhesion molecules during development of the cerebral cortex
T2 - the J1 /tenascin glycoprotein in the mouse somatosensory cortical barrel field
AU - Steindler, Dennis A.
AU - Cooper, Nigel G.F.
AU - Faissner, Andreas
AU - Schachner, Melitta
N1 - Funding Information:
This work would not have been possible without the contributions of Kristine Harrington, Sharon FraNc, Marvinetta Cooper, HuN Kuo, Tom O'Brien, Budd Jackson, Meyer Horn, Stephen Peterson, and Darlene Warford. We are grateful for insightful discussions with Dr. Roger Markwald during the course of these studies. This work was supported by NIH Grants NS 20856 from the NINCDS (DAN), EY 02708 from the NEI (NGFC), and the Deutsche Forschungsgemein-schaft (AF and MS).
PY - 1989/1
Y1 - 1989/1
N2 - The distribution of the 200/220 KDa J1 glycoprotein (Jl-200/220), within the developing vibrissae-related barrel field of the mouse somatosensory cortex, was studied by immunocytochemistry using a monoclonal antibody. JI-200/220, a member of the L2/HNK-1 family of adhesion molecules, also appears to be the mouse homologue of tenascin. Jl/tenascin-positive barrel-like structures are visible in the somatosensory cortex between 24 and 48 hr after birth, with the molecule present in prospective barrel boundaries. Immunoelectronmicroscopy reveals labeling that is associated with glial and neuronal plasma membranes, as well as glial end-feet on blood vessels. A possible major source of Jl/tenascin expression at this time is astrocyte precursor cells and radial glia. In the putative astrocyte precursor cells, immunolabeling was observed within organelles including the Golgi apparatus. At P6-7 Jl/tenascin is most prevalent within prospective interbarrel septae. Jl/tenascin-positive barrel boundaries are barely visible on P9 and not observed on P16. The findings indicate that Jl/tenascin represents a major component of previously described "hidden" boundaries that we have seen during development using other methodologies. The expression of adhesion molecule-rich boundaries during the critical stages of barrel field formation indicates roles for such molecules during specific cerebral cortical pattern formation events.
AB - The distribution of the 200/220 KDa J1 glycoprotein (Jl-200/220), within the developing vibrissae-related barrel field of the mouse somatosensory cortex, was studied by immunocytochemistry using a monoclonal antibody. JI-200/220, a member of the L2/HNK-1 family of adhesion molecules, also appears to be the mouse homologue of tenascin. Jl/tenascin-positive barrel-like structures are visible in the somatosensory cortex between 24 and 48 hr after birth, with the molecule present in prospective barrel boundaries. Immunoelectronmicroscopy reveals labeling that is associated with glial and neuronal plasma membranes, as well as glial end-feet on blood vessels. A possible major source of Jl/tenascin expression at this time is astrocyte precursor cells and radial glia. In the putative astrocyte precursor cells, immunolabeling was observed within organelles including the Golgi apparatus. At P6-7 Jl/tenascin is most prevalent within prospective interbarrel septae. Jl/tenascin-positive barrel boundaries are barely visible on P9 and not observed on P16. The findings indicate that Jl/tenascin represents a major component of previously described "hidden" boundaries that we have seen during development using other methodologies. The expression of adhesion molecule-rich boundaries during the critical stages of barrel field formation indicates roles for such molecules during specific cerebral cortical pattern formation events.
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U2 - 10.1016/S0012-1606(89)80056-9
DO - 10.1016/S0012-1606(89)80056-9
M3 - Article
C2 - 2462518
AN - SCOPUS:0024535146
SN - 0012-1606
VL - 131
SP - 243
EP - 260
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -