Branched RNA covalently linked to the 5′ end of a single-stranded DNA in Stigmatella aurantiaca: Structure of msDNA

Teiichi Furuichi; Anil Dhundale; Masayori Inouye; Sumiko Inouye

doi:10.1016/0092-8674(87)90354-0

Branched RNA covalently linked to the 5′ end of a single-stranded DNA in Stigmatella aurantiaca: Structure of msDNA

Teiichi Furuichi, Anil Dhundale, Masayori Inouye, Sumiko Inouye

Research output: Contribution to journal › Article › peer-review

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Abstract

Stigmatella aurantiaca is a gliding, gram-negative bacterium that shows a spectacular fruiting body formation upon starvation of nutrient. This bacterium was found to contain approximately 500 copies per cell of a short single-stranded linear DNA (multicopy single-stranded DNA: msDNA). The primary structure of msDNA was determined and found to consist of 162 or 163 deoxyrlbonucleotides. Its unique chromosomal gene was cloned and sequenced. The msDNA was found to be attached to a branched RNA by its 5′ end. Structural analysis of the branched RNA revealed that it consists of a triribonucleotide, ^5′A-G-(C or U)^3′, and that msDNA is branched out from the 2′ position of the rG residue forming a 2′, 5′ phosphodiester linkage with the dC residue at the 5′ end of msDNA.

Original language	English (US)
Pages (from-to)	47-53
Number of pages	7
Journal	Cell
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(87)90354-0
State	Published - Jan 16 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(87)90354-0

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@article{2537f420ee7f464ebd7ec09ed664d21b,

title = "Branched RNA covalently linked to the 5′ end of a single-stranded DNA in Stigmatella aurantiaca: Structure of msDNA",

abstract = "Stigmatella aurantiaca is a gliding, gram-negative bacterium that shows a spectacular fruiting body formation upon starvation of nutrient. This bacterium was found to contain approximately 500 copies per cell of a short single-stranded linear DNA (multicopy single-stranded DNA: msDNA). The primary structure of msDNA was determined and found to consist of 162 or 163 deoxyrlbonucleotides. Its unique chromosomal gene was cloned and sequenced. The msDNA was found to be attached to a branched RNA by its 5′ end. Structural analysis of the branched RNA revealed that it consists of a triribonucleotide, 5′A-G-(C or U)3′, and that msDNA is branched out from the 2′ position of the rG residue forming a 2′, 5′ phosphodiester linkage with the dC residue at the 5′ end of msDNA.",

author = "Teiichi Furuichi and Anil Dhundale and Masayori Inouye and Sumiko Inouye",

note = "Funding Information: We would like to thank Dr. Danny Reinberg for his valuable discussion for the determination of the branched RNA structure, and Drs. Jaime Arenas and Jerard Hurwitz for making debranching enzyme and information concerning the enzyme available to us before publication. We would also like to thank Drs. 8. Lampson and T. Komano for critical reading of the manuscript. The work was supported by Public Health Service grant GM26843 The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.",

year = "1987",

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doi = "10.1016/0092-8674(87)90354-0",

language = "English (US)",

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AU - Dhundale, Anil

AU - Inouye, Masayori

AU - Inouye, Sumiko

N1 - Funding Information: We would like to thank Dr. Danny Reinberg for his valuable discussion for the determination of the branched RNA structure, and Drs. Jaime Arenas and Jerard Hurwitz for making debranching enzyme and information concerning the enzyme available to us before publication. We would also like to thank Drs. 8. Lampson and T. Komano for critical reading of the manuscript. The work was supported by Public Health Service grant GM26843 The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

PY - 1987/1/16

Y1 - 1987/1/16

N2 - Stigmatella aurantiaca is a gliding, gram-negative bacterium that shows a spectacular fruiting body formation upon starvation of nutrient. This bacterium was found to contain approximately 500 copies per cell of a short single-stranded linear DNA (multicopy single-stranded DNA: msDNA). The primary structure of msDNA was determined and found to consist of 162 or 163 deoxyrlbonucleotides. Its unique chromosomal gene was cloned and sequenced. The msDNA was found to be attached to a branched RNA by its 5′ end. Structural analysis of the branched RNA revealed that it consists of a triribonucleotide, 5′A-G-(C or U)3′, and that msDNA is branched out from the 2′ position of the rG residue forming a 2′, 5′ phosphodiester linkage with the dC residue at the 5′ end of msDNA.

AB - Stigmatella aurantiaca is a gliding, gram-negative bacterium that shows a spectacular fruiting body formation upon starvation of nutrient. This bacterium was found to contain approximately 500 copies per cell of a short single-stranded linear DNA (multicopy single-stranded DNA: msDNA). The primary structure of msDNA was determined and found to consist of 162 or 163 deoxyrlbonucleotides. Its unique chromosomal gene was cloned and sequenced. The msDNA was found to be attached to a branched RNA by its 5′ end. Structural analysis of the branched RNA revealed that it consists of a triribonucleotide, 5′A-G-(C or U)3′, and that msDNA is branched out from the 2′ position of the rG residue forming a 2′, 5′ phosphodiester linkage with the dC residue at the 5′ end of msDNA.

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Branched RNA covalently linked to the 5′ end of a single-stranded DNA in Stigmatella aurantiaca: Structure of msDNA

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