Brief report: Interferon-γ induces expansion of Lin 2Sca-1+C-Kit+ cells

Xin Zhao, Guangwen Ren, Li Liang, Phillip Z. Ai, Betty Zheng, Jay A. Tischfield, Yufang Shi, Changshun Shao

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The balance between Th1 and Th2 cells is critical for homeostasis of the immune system. Th1 cells can also regulate hematopoietic progenitor cell homeostasis by production of oncostatin M. Here we show that Th1 cell products, but not those of Th2 cells, caused a rapid expansion of lineage -Sca-1+C-kit+ (LSK) cells in vivo and in vitro. Among Th1 cytokines, interferon-γ (IFNγ) was found to play a major role in this expansion by activating the expression of Sca-1 in lineage -Sca-1-C-kit+ cells. This process was dependent on IFNγR1 signaling and the STAT1 pathway. Furthermore, those IFNγ-induced LSK cells had a higher proliferation potential than control LSK cells. In addition, while the over-all production of colony-forming units in bone marrow was decreased after IFNγ treatment, the sorted LSK cells could give rise to a higher yield of colony-forming units. Finally, the IFNγ-induced hematopoiesis was biased toward the differentiation of myeloid lineages. Therefore, our findings demonstrated a novel role of IFNγ in activating hematopoietic progenitor cells and provide a new insight into the clinical application of interferon.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalSTEM CELLS
Volume28
Issue number1
DOIs
StatePublished - Jan 2010

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Keywords

  • Hematopoietic progenitor cells
  • Interferon-γ
  • Lineage
  • Lineage Sca-1C-kit cells

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