Calcium phosphate transfection of primary hippocampal neurons.

Miao Sun; Laura P. Bernard; Victoria L. Dibona; Qian Wu; Huaye Zhang

doi:10.3791/50808

Calcium phosphate transfection of primary hippocampal neurons.

Miao Sun, Laura P. Bernard, Victoria L. Dibona, Qian Wu, Huaye Zhang

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

Original language	English (US)
Pages (from-to)	e50808
Journal	Journal of visualized experiments : JoVE
Issue number	81
DOIs	https://doi.org/10.3791/50808
State	Published - 2013

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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title = "Calcium phosphate transfection of primary hippocampal neurons.",

abstract = "Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.",

author = "Miao Sun and Bernard, {Laura P.} and Dibona, {Victoria L.} and Qian Wu and Huaye Zhang",

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AU - Wu, Qian

AU - Zhang, Huaye

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