Calcium phosphate transfection of primary hippocampal neurons.

Miao Sun, Laura P. Bernard, Victoria L. Dibona, Qian Wu, Huaye Zhang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

Original languageEnglish (US)
Pages (from-to)e50808
JournalJournal of visualized experiments : JoVE
Issue number81
DOIs
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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