cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF2α synthesis in mouse hepatocytes

József Mandl; István Mucha; Gábor Bánhegyi; György Mészáros; Anna Faragó; Zoltán Spolarics; Raymund Machovich; Ferenc Antoni; Tamás Garzó

doi:10.1016/0090-6980(88)90054-8

cAMP dependent inhibition of thromboxane A₂, prostacyclin and PGF_2α synthesis in mouse hepatocytes

József Mandl, István Mucha, Gábor Bánhegyi, György Mészáros, Anna Faragó, Zoltán Spolarics, Raymund Machovich, Ferenc Antoni, Tamás Garzó

Research output: Contribution to journal › Article › peer-review

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Abstract

The role of cAMP dependent regulation in thromboxane A₂, prostacyclin and PGF_2α synthesis (measured by radioimmunoassay) was investigated in isolated mouse hepatocytes and in microsomal membranes prepared from these cells. In isolated hepatocytes N⁶,O²-dibutyryl cAMP inhibited the formation of all the three derivatives, while calcium ionophore A 23187 stimulated their synthesis. Addition of the dissociated catalytic subunit of cAMP dependent protein kinase and ATP to microsomal membranes inhibited the production of TXA₂, PGI₂ and PGF_2α by about 50% and this inhibition was counteracted by the combined addition of heat stable inhibitor protein of cAMP dependent protein kinase. It is concluded that in parenchylmal liver cells cAMP dependent phosphorylation is directly involved in the inhibition of prostanoid synthesis.

Original language	English (US)
Pages (from-to)	761-772
Number of pages	12
Journal	Prostaglandins
Volume	36
Issue number	6
DOIs	https://doi.org/10.1016/0090-6980(88)90054-8
State	Published - Dec 1988
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Endocrinology

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10.1016/0090-6980(88)90054-8

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title = "cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF2α synthesis in mouse hepatocytes",

abstract = "The role of cAMP dependent regulation in thromboxane A2, prostacyclin and PGF2α synthesis (measured by radioimmunoassay) was investigated in isolated mouse hepatocytes and in microsomal membranes prepared from these cells. In isolated hepatocytes N6,O2-dibutyryl cAMP inhibited the formation of all the three derivatives, while calcium ionophore A 23187 stimulated their synthesis. Addition of the dissociated catalytic subunit of cAMP dependent protein kinase and ATP to microsomal membranes inhibited the production of TXA2, PGI2 and PGF2α by about 50% and this inhibition was counteracted by the combined addition of heat stable inhibitor protein of cAMP dependent protein kinase. It is concluded that in parenchylmal liver cells cAMP dependent phosphorylation is directly involved in the inhibition of prostanoid synthesis.",

author = "J{\'o}zsef Mandl and Istv{\'a}n Mucha and G{\'a}bor B{\'a}nhegyi and Gy{\"o}rgy M{\'e}sz{\'a}ros and Anna Farag{\'o} and Zolt{\'a}n Spolarics and Raymund Machovich and Ferenc Antoni and Tam{\'a}s Garz{\'o}",

note = "Funding Information: Thanks are due to Mrs. Gizella Ferencz and Ms. Jolan Fazekas for skillful technical assistance, This work was supported by the Hungarian Academyo f Sciences (l-3-86-329).",

year = "1988",

month = dec,

doi = "10.1016/0090-6980(88)90054-8",

language = "English (US)",

volume = "36",

pages = "761--772",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier BV",

number = "6",

}

TY - JOUR

T1 - cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF2α synthesis in mouse hepatocytes

AU - Mandl, József

AU - Mucha, István

AU - Bánhegyi, Gábor

AU - Mészáros, György

AU - Faragó, Anna

AU - Spolarics, Zoltán

AU - Machovich, Raymund

AU - Antoni, Ferenc

AU - Garzó, Tamás

N1 - Funding Information: Thanks are due to Mrs. Gizella Ferencz and Ms. Jolan Fazekas for skillful technical assistance, This work was supported by the Hungarian Academyo f Sciences (l-3-86-329).

PY - 1988/12

Y1 - 1988/12

N2 - The role of cAMP dependent regulation in thromboxane A2, prostacyclin and PGF2α synthesis (measured by radioimmunoassay) was investigated in isolated mouse hepatocytes and in microsomal membranes prepared from these cells. In isolated hepatocytes N6,O2-dibutyryl cAMP inhibited the formation of all the three derivatives, while calcium ionophore A 23187 stimulated their synthesis. Addition of the dissociated catalytic subunit of cAMP dependent protein kinase and ATP to microsomal membranes inhibited the production of TXA2, PGI2 and PGF2α by about 50% and this inhibition was counteracted by the combined addition of heat stable inhibitor protein of cAMP dependent protein kinase. It is concluded that in parenchylmal liver cells cAMP dependent phosphorylation is directly involved in the inhibition of prostanoid synthesis.

AB - The role of cAMP dependent regulation in thromboxane A2, prostacyclin and PGF2α synthesis (measured by radioimmunoassay) was investigated in isolated mouse hepatocytes and in microsomal membranes prepared from these cells. In isolated hepatocytes N6,O2-dibutyryl cAMP inhibited the formation of all the three derivatives, while calcium ionophore A 23187 stimulated their synthesis. Addition of the dissociated catalytic subunit of cAMP dependent protein kinase and ATP to microsomal membranes inhibited the production of TXA2, PGI2 and PGF2α by about 50% and this inhibition was counteracted by the combined addition of heat stable inhibitor protein of cAMP dependent protein kinase. It is concluded that in parenchylmal liver cells cAMP dependent phosphorylation is directly involved in the inhibition of prostanoid synthesis.

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U2 - 10.1016/0090-6980(88)90054-8

DO - 10.1016/0090-6980(88)90054-8

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C2 - 2854284

AN - SCOPUS:0024240509

SN - 0090-6980

VL - 36

SP - 761

EP - 772

JO - Prostaglandins

JF - Prostaglandins

IS - 6

ER -

cAMP dependent inhibition of thromboxane A₂, prostacyclin and PGF_2α synthesis in mouse hepatocytes

Abstract

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