cAMP dependent inhibition of thromboxane A2, prostacyclin and PGF synthesis in mouse hepatocytes

József Mandl, István Mucha, Gábor Bánhegyi, György Mészáros, Anna Faragó, Zoltán Spolarics, Raymund Machovich, Ferenc Antoni, Tamás Garzó

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5 Scopus citations

Abstract

The role of cAMP dependent regulation in thromboxane A2, prostacyclin and PGF synthesis (measured by radioimmunoassay) was investigated in isolated mouse hepatocytes and in microsomal membranes prepared from these cells. In isolated hepatocytes N6,O2-dibutyryl cAMP inhibited the formation of all the three derivatives, while calcium ionophore A 23187 stimulated their synthesis. Addition of the dissociated catalytic subunit of cAMP dependent protein kinase and ATP to microsomal membranes inhibited the production of TXA2, PGI2 and PGF by about 50% and this inhibition was counteracted by the combined addition of heat stable inhibitor protein of cAMP dependent protein kinase. It is concluded that in parenchylmal liver cells cAMP dependent phosphorylation is directly involved in the inhibition of prostanoid synthesis.

Original languageEnglish (US)
Pages (from-to)761-772
Number of pages12
JournalProstaglandins
Volume36
Issue number6
DOIs
StatePublished - Dec 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

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