cAMP-dependent protein kinase modulation of glycine-activated chloride current in neurons freshly isolated from rat ventral tegmental area

Jun Ren, Jiang Hong Ye, Joseph J. McArdle

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Adenosine 3',5'cyclic monophosphate-(cAMP)-dependent protein kinase (PKA) modulation of glycine-activated Cl- currents (I(Gly)) in single neurons freshly isolated from the rat ventral tegmental area (VTA) was studied using whole-cell patch-clamp technique. In the majority of cells tested with Mg-ATP in the internal solution, I(Gly) induced by 3-10 μM glycine increased spontaneously (ran up). In the absence of internal ATP, I(Gly) remained stable in six of seven cells. External perfusion of 8-Br- cAMP, a PKA activator, potentiated I(Gly) only in cells showing run-up. 8- Br-cAMP potentiated I(Gly) induced by low concentrations of glycine, but had no effect on the maximal current. When added to the pipette solution, H-89, a PKA inhibitor, blocked ATP and 8-Br-cAMP induced run-up of I(Gly). In contrast, dialysis with chelerythrine, a PKC inhibitor, did not alter the run-up of I(Gly). These results suggest that the PKA pathway modulates the activity of the glycine receptor/channel complex via enhancing the affinity of the receptor for glycine.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalBrain research
Volume811
Issue number1-2
DOIs
StatePublished - Nov 16 1998

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • ATP
  • Ion channel
  • Patch clamp
  • Phosphorylation

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