Campylobacter pylori colonization factor shows specificity for lactosylceramide sulfate and GM3 ganglioside

B. L. Slomiany, J. Piotrowski, A. Samanta, K. VanHorn, V. L.N. Murty, A. Slomiany

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

The specificity of Campylobacter pylori cell surface lectin, a presumptive colonization factor, was investigated using various sulfated and sialic acid containing glycolipids. C. pylori cells, cultured from human antral mucosal biopsies, were incubated with intact and modified glycolipid preparations and examined for agglutination inhibition of human erythrocytes. Titration data revealed that the inhibitory activity was highest with lactosylceramide sulfate and GM3 ganglioside, while galactosyl-ceramide sulfate GM1, GD1a and GD1b gangliosides were less effective. A strong inhibitory activity towards C. pylori hemagglutin was also observed with an antiulcer agent, sucralfate. The inhibitory effect of both types of glycolipids was abolished by the removal of sialic acid and sulfate ester groups, thus indicating that sulfated and sialic acid containing glycolipids with terminal lactosyl moieties serve as mucosal receptors for colonization of gastric epithelium by C. pylori.

Original languageEnglish (US)
Pages (from-to)929-936
Number of pages8
JournalBiochemistry International
Volume19
Issue number4
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry

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