Caspofungin uptake is mediated by a high-affinity transporter in Candida albicans

Padmaja Paderu, Steven Park, David S. Perlin

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The uptake of the echinocandin drug caspofungin acetate in Candida albicans was evaluated at drug levels at or near the MIC for the organism. Maximal uptake was achieved in 10 min and was energy independent. A saturable transport system, consistent with a facilitated-diffusion carrier, was observed with the unlabeled drug competing with the labeled drug for uptake and efflux. More than 90% of the transported drug was observed in a single kinetic compartment that was available for efflux, indicating that the drug was free in the cytoplasm following uptake. Efflux was also energy independent but was sensitive to the presence of a fully loaded carrier on both faces of the bilayer. Overall, the data presented are consistent with the presence of a high-affinity facilitated-diffusion transporter that mediates caspofungin uptake and could be a potential source of transport-related reduced susceptibility.

Original languageEnglish (US)
Pages (from-to)3845-3849
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume48
Issue number10
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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