Cation conductance regulated by ambient cl in cultured rat mesangial cells?

Chien Jung Huang, Carlos E. Palant, William Craelius

Research output: Contribution to journalArticle

Abstract

Several functions of mesangial cells, such as contraction and release of nitric oxide, are dependent on the ambient Cl concentration. Herein we describe a direct effect of Cl on mesangial cell membrane conductance. Rat mesangial cells were isolated, cultured, and were patch-clamped in the whole-cell configuration. The external concentration – ([Cl]o) – of standard Ringer’s solutions was changed isotonically from standard of 160 mM to 35 mM while cell membrane conductance was continuously recorded. Results indicated that control I-V curves of the steady state current had a linear slope conductance of 0.95 nS (n = 10, r = 0.9). In low [Cl]o, conductance outwardly rectified and increased to 4.1 nS between -120 and 0 mV and to 16.8 nS between 0 and 40 mV (n = 10, r = 0.9;p < 0.01).The increase in conductance occurred within a few seconds and was reversible. Our results show that a large mesangial cell membrane conductance for cations is activated upon exposure to low [Cl]o.

Original languageEnglish (US)
Pages (from-to)302-306
Number of pages5
JournalKidney and Blood Pressure Research
Volume20
Issue number5
DOIs
StatePublished - Jan 1 1997

Fingerprint

Mesangial Cells
Cations
Cell Membrane
Nitric Oxide

All Science Journal Classification (ASJC) codes

  • Nephrology
  • Cardiology and Cardiovascular Medicine

Keywords

  • Chloride
  • Ion current
  • Mesangial cells

Cite this

@article{d91d2b1162244650946474d495fe0750,
title = "Cation conductance regulated by ambient cl− in cultured rat mesangial cells?",
abstract = "Several functions of mesangial cells, such as contraction and release of nitric oxide, are dependent on the ambient Cl− concentration. Herein we describe a direct effect of Cl− on mesangial cell membrane conductance. Rat mesangial cells were isolated, cultured, and were patch-clamped in the whole-cell configuration. The external concentration – ([Cl−]o) – of standard Ringer’s solutions was changed isotonically from standard of 160 mM to 35 mM while cell membrane conductance was continuously recorded. Results indicated that control I-V curves of the steady state current had a linear slope conductance of 0.95 nS (n = 10, r = 0.9). In low [Cl−]o, conductance outwardly rectified and increased to 4.1 nS between -120 and 0 mV and to 16.8 nS between 0 and 40 mV (n = 10, r = 0.9;p < 0.01).The increase in conductance occurred within a few seconds and was reversible. Our results show that a large mesangial cell membrane conductance for cations is activated upon exposure to low [Cl−]o.",
keywords = "Chloride, Ion current, Mesangial cells",
author = "Huang, {Chien Jung} and Palant, {Carlos E.} and William Craelius",
year = "1997",
month = "1",
day = "1",
doi = "10.1159/000174163",
language = "English (US)",
volume = "20",
pages = "302--306",
journal = "Kidney and Blood Pressure Research",
issn = "1420-4096",
publisher = "S. Karger AG",
number = "5",

}

Cation conductance regulated by ambient cl in cultured rat mesangial cells? / Huang, Chien Jung; Palant, Carlos E.; Craelius, William.

In: Kidney and Blood Pressure Research, Vol. 20, No. 5, 01.01.1997, p. 302-306.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cation conductance regulated by ambient cl− in cultured rat mesangial cells?

AU - Huang, Chien Jung

AU - Palant, Carlos E.

AU - Craelius, William

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Several functions of mesangial cells, such as contraction and release of nitric oxide, are dependent on the ambient Cl− concentration. Herein we describe a direct effect of Cl− on mesangial cell membrane conductance. Rat mesangial cells were isolated, cultured, and were patch-clamped in the whole-cell configuration. The external concentration – ([Cl−]o) – of standard Ringer’s solutions was changed isotonically from standard of 160 mM to 35 mM while cell membrane conductance was continuously recorded. Results indicated that control I-V curves of the steady state current had a linear slope conductance of 0.95 nS (n = 10, r = 0.9). In low [Cl−]o, conductance outwardly rectified and increased to 4.1 nS between -120 and 0 mV and to 16.8 nS between 0 and 40 mV (n = 10, r = 0.9;p < 0.01).The increase in conductance occurred within a few seconds and was reversible. Our results show that a large mesangial cell membrane conductance for cations is activated upon exposure to low [Cl−]o.

AB - Several functions of mesangial cells, such as contraction and release of nitric oxide, are dependent on the ambient Cl− concentration. Herein we describe a direct effect of Cl− on mesangial cell membrane conductance. Rat mesangial cells were isolated, cultured, and were patch-clamped in the whole-cell configuration. The external concentration – ([Cl−]o) – of standard Ringer’s solutions was changed isotonically from standard of 160 mM to 35 mM while cell membrane conductance was continuously recorded. Results indicated that control I-V curves of the steady state current had a linear slope conductance of 0.95 nS (n = 10, r = 0.9). In low [Cl−]o, conductance outwardly rectified and increased to 4.1 nS between -120 and 0 mV and to 16.8 nS between 0 and 40 mV (n = 10, r = 0.9;p < 0.01).The increase in conductance occurred within a few seconds and was reversible. Our results show that a large mesangial cell membrane conductance for cations is activated upon exposure to low [Cl−]o.

KW - Chloride

KW - Ion current

KW - Mesangial cells

UR - http://www.scopus.com/inward/record.url?scp=0030782705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030782705&partnerID=8YFLogxK

U2 - 10.1159/000174163

DO - 10.1159/000174163

M3 - Article

C2 - 9419045

AN - SCOPUS:0030782705

VL - 20

SP - 302

EP - 306

JO - Kidney and Blood Pressure Research

JF - Kidney and Blood Pressure Research

SN - 1420-4096

IS - 5

ER -