Cation conductance regulated by ambient cl in cultured rat mesangial cells?

Chien Jung Huang, Carlos E. Palant, William Craelius

Research output: Contribution to journalArticle

Abstract

Several functions of mesangial cells, such as contraction and release of nitric oxide, are dependent on the ambient Cl concentration. Herein we describe a direct effect of Cl on mesangial cell membrane conductance. Rat mesangial cells were isolated, cultured, and were patch-clamped in the whole-cell configuration. The external concentration – ([Cl]o) – of standard Ringer’s solutions was changed isotonically from standard of 160 mM to 35 mM while cell membrane conductance was continuously recorded. Results indicated that control I-V curves of the steady state current had a linear slope conductance of 0.95 nS (n = 10, r = 0.9). In low [Cl]o, conductance outwardly rectified and increased to 4.1 nS between -120 and 0 mV and to 16.8 nS between 0 and 40 mV (n = 10, r = 0.9;p < 0.01).The increase in conductance occurred within a few seconds and was reversible. Our results show that a large mesangial cell membrane conductance for cations is activated upon exposure to low [Cl]o.

Original languageEnglish (US)
Pages (from-to)302-306
Number of pages5
JournalKidney and Blood Pressure Research
Volume20
Issue number5
DOIs
StatePublished - Jan 1 1997

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Nephrology
  • Cardiology and Cardiovascular Medicine

Keywords

  • Chloride
  • Ion current
  • Mesangial cells

Cite this