CD28 Dependence of T Cell Differentiation to IL-4 Production Varies with the Particular Type 2 Immune Response

William C. Gause, Shen Jue Chen, Rebecca J. Greenwald, Mark J. Halvorson, Pin Lu, Xia Di Zhou, Suzanne C. Morris, Kelvin P. Lee, Carl H. June, Fred D. Finkelman, Joseph F. Urban, Ryo Abe

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

T cell differentiation to effector cell function is required for the development of a type 2 immune response. The T cell surface molecule, CD28, is widely considered to be the principal costimulatory molecule involved in T cell differentiation to effector function, including IL-4 production, although this has been difficult to directly examine in vivo. We have studied in vivo differentiation to T cell effector function during two type 2 immune responses in CD28 knockout mice: the systemic immune response to goat anti-mouse IgD Ab and the mucosal immune response following oral inoculation with the nematode parasite, Heligmosomoides polygyrus. Our results show that in C57BL/6 CD28 knockout mice elevations in IL-4 gene expression and protein secretion are blocked during the immune response to goat anti-mouse IgD, and associated increases in serum IgG1 and IgE are also inhibited to untreated control levels. In marked contrast, T cell differentiation to IL-4 production is comparable in C57BL/6 CD28 -/- and CD28 +/+ H. polygyrus-inoculated mice, and elevations in both serum IgG1 and IgE levels occur. These results indicate that the specific kind of type 2 immune response determines whether T cell differentiation to IL-4 production is CD28 dependent.

Original languageEnglish (US)
Pages (from-to)4082-4087
Number of pages6
JournalJournal of Immunology
Volume158
Issue number9
StatePublished - May 1 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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