CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Lilianne Barbar; Tanya Jain; Matthew Zimmer; Ilya Kruglikov; Jessica S. Sadick; Minghui Wang; Kriti Kalpana; Indigo V.L. Rose; Suzanne R. Burstein; Tomasz Rusielewicz; Madhura Nijsure; Kevin A. Guttenplan; Angelique di Domenico; Gist Croft; Bin Zhang; Hiroko Nobuta; Jean M. Hébert; Shane A. Liddelow; Valentina Fossati

doi:10.1016/j.neuron.2020.05.014

CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Lilianne Barbar, Tanya Jain, Matthew Zimmer, Ilya Kruglikov, Jessica S. Sadick, Minghui Wang, Kriti Kalpana, Indigo V.L. Rose, Suzanne R. Burstein, Tomasz Rusielewicz, Madhura Nijsure, Kevin A. Guttenplan, Angelique di Domenico, Gist Croft, Bin Zhang, Hiroko Nobuta, Jean M. Hébert, Shane A. Liddelow, Valentina Fossati

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

Barbar et al. identify CD49f as a novel surface marker expressed by human astrocytes that can purify hiPSC-astrocytes and primary fetal astrocytes. CD49f⁺ hiPSC-astrocytes respond to pro-inflammatory stimuli and become A1 reactive astrocytes, which are dysfunctional and secrete neurotoxic factors that induce apoptosis in human and rodent neurons.

Original language	English (US)
Pages (from-to)	436-453.e12
Journal	Neuron
Volume	107
Issue number	3
DOIs	https://doi.org/10.1016/j.neuron.2020.05.014
State	Published - Aug 5 2020
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

Keywords

A1 reactive astrocytes
CD49f
FACS purification
astrocytes
induced pluripotent stem cells
neurodegeneration
neurotoxicity

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10.1016/j.neuron.2020.05.014

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Barbar, L., Jain, T., Zimmer, M., Kruglikov, I., Sadick, J. S., Wang, M., Kalpana, K., Rose, I. V. L., Burstein, S. R., Rusielewicz, T., Nijsure, M., Guttenplan, K. A., di Domenico, A., Croft, G., Zhang, B., Nobuta, H., Hébert, J. M., Liddelow, S. A., & Fossati, V. (2020). CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes. Neuron, 107(3), 436-453.e12. https://doi.org/10.1016/j.neuron.2020.05.014

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title = "CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes",

abstract = "Barbar et al. identify CD49f as a novel surface marker expressed by human astrocytes that can purify hiPSC-astrocytes and primary fetal astrocytes. CD49f+ hiPSC-astrocytes respond to pro-inflammatory stimuli and become A1 reactive astrocytes, which are dysfunctional and secrete neurotoxic factors that induce apoptosis in human and rodent neurons.",

keywords = "A1 reactive astrocytes, CD49f, FACS purification, astrocytes, induced pluripotent stem cells, neurodegeneration, neurotoxicity",

author = "Lilianne Barbar and Tanya Jain and Matthew Zimmer and Ilya Kruglikov and Sadick, {Jessica S.} and Minghui Wang and Kriti Kalpana and Rose, {Indigo V.L.} and Burstein, {Suzanne R.} and Tomasz Rusielewicz and Madhura Nijsure and Guttenplan, {Kevin A.} and {di Domenico}, Angelique and Gist Croft and Bin Zhang and Hiroko Nobuta and H{\'e}bert, {Jean M.} and Liddelow, {Shane A.} and Valentina Fossati",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = aug,

day = "5",

doi = "10.1016/j.neuron.2020.05.014",

language = "English (US)",

volume = "107",

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Barbar, L, Jain, T, Zimmer, M, Kruglikov, I, Sadick, JS, Wang, M, Kalpana, K, Rose, IVL, Burstein, SR, Rusielewicz, T, Nijsure, M, Guttenplan, KA, di Domenico, A, Croft, G, Zhang, B, Nobuta, H, Hébert, JM, Liddelow, SA & Fossati, V 2020, 'CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes', Neuron, vol. 107, no. 3, pp. 436-453.e12. https://doi.org/10.1016/j.neuron.2020.05.014

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T1 - CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

AU - Barbar, Lilianne

AU - Jain, Tanya

AU - Zimmer, Matthew

AU - Kruglikov, Ilya

AU - Sadick, Jessica S.

AU - Wang, Minghui

AU - Kalpana, Kriti

AU - Rose, Indigo V.L.

AU - Burstein, Suzanne R.

AU - Rusielewicz, Tomasz

AU - Nijsure, Madhura

AU - Guttenplan, Kevin A.

AU - di Domenico, Angelique

AU - Croft, Gist

AU - Zhang, Bin

AU - Nobuta, Hiroko

AU - Hébert, Jean M.

AU - Liddelow, Shane A.

AU - Fossati, Valentina

PY - 2020/8/5

Y1 - 2020/8/5

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AB - Barbar et al. identify CD49f as a novel surface marker expressed by human astrocytes that can purify hiPSC-astrocytes and primary fetal astrocytes. CD49f+ hiPSC-astrocytes respond to pro-inflammatory stimuli and become A1 reactive astrocytes, which are dysfunctional and secrete neurotoxic factors that induce apoptosis in human and rodent neurons.

KW - A1 reactive astrocytes

KW - CD49f

KW - FACS purification

KW - astrocytes

KW - induced pluripotent stem cells

KW - neurodegeneration

KW - neurotoxicity

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JO - Neuron

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ER -

CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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