Cell-specific patterns of oscillating free Ca2+ in carbamylcholine-stimulated insulinoma cells

M. Prentki, M. C. Glennon, A. P. Thomas, R. L. Morris, F. M. Matschinsky, B. E. Corkey

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Abstract

The effect of the muscarinic agonist carbamylcholine on cytoplasmic Ca2+ concentration ([Ca2+](i) was examined at the single cell level in clonal pancreatic β-cells (HIT). Cells were loaded with the indicator dye fura 2, and [Ca2+](i) was measured by microfluorimetry. Carbamylcholine induced changes in Ca2+ that differed from cell to cell and provoked in some cells oscillatory Ca2+ fluctuations. During a transient, free Ca2+ rose to a peak within 1-3 s. The frequency of the oscillations increased with agonist concentration. Oscillations in [Ca2+](i) occurred in the absence of external Ca2+. When cells were perifused for a sufficient period of time without carbamylcholine, near identical Ca2+ responses were elicited in each cell by successive applications of the agonist. Thus, individual cells displayed characteristic and reproducible Ca2+ responses with respect to amplitude, frequency, and shape of the transients as well as latency in onset of the initial Ca2+ rise. We propose that the biological response to a Ca2+ agonist in a given cell is not only determined by the frequency and amplitude of Ca2+ oscillations but is governed by the unique pattern of the Ca2+ signal of each cell, which may be termed 'Ca2+ fingerprint'.

Original languageEnglish (US)
Pages (from-to)11044-11047
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number23
StatePublished - 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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