Cell type-specific structural plasticity of the ciliary transition zone in C. elegans

Jyothi S. Akella, Malan Silva, Natalia S. Morsci, Ken C. Nguyen, William J. Rice, David H. Hall, Maureen Barr

Research output: Contribution to journalArticle

Abstract

Background information: The current consensus on cilia development posits that the ciliary transition zone (TZ) is formed via extension of nine centrosomal microtubules. In this model, TZ structure remains unchanged in microtubule number throughout the cilium life cycle. This model does not however explain structural variations of TZ structure seen in nature and could also lend itself to the misinterpretation that deviations from nine-doublet microtubule ultrastructure represent an abnormal phenotype. Thus, a better understanding of events that occur at the TZ in vivo during metazoan development is required. Results: To address this issue, we characterized ultrastructure of two types of sensory cilia in developing Caenorhabditis elegans. We discovered that, in cephalic male (CEM) and inner labial quadrant (IL2Q) sensory neurons, ciliary TZs are structurally plastic and remodel from one structure to another during animal development. The number of microtubule doublets forming the TZ can be increased or decreased over time, depending on cilia type. Both cases result in structural TZ intermediates different from TZ in cilia of adult animals. In CEM cilia, axonemal extension and maturation occurs concurrently with TZ structural maturation. Conclusions and Significance: Our work extends the current model to include the structural plasticity of metazoan transition zone, which can be structurally delayed, maintained or remodelled in cell type-specific manner.

Original languageEnglish (US)
Pages (from-to)95-107
Number of pages13
JournalBiology of the Cell
Volume111
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Cilia
Microtubules
Head
Caenorhabditis elegans
Sensory Receptor Cells
Lip
Life Cycle Stages
Plastics
Phenotype

All Science Journal Classification (ASJC) codes

  • Cell Biology

Keywords

  • C. elegans
  • axoneme
  • cilia
  • microtubule
  • transition zone

Cite this

Akella, J. S., Silva, M., Morsci, N. S., Nguyen, K. C., Rice, W. J., Hall, D. H., & Barr, M. (2019). Cell type-specific structural plasticity of the ciliary transition zone in C. elegans. Biology of the Cell, 111(4), 95-107. https://doi.org/10.1111/boc.201800042
Akella, Jyothi S. ; Silva, Malan ; Morsci, Natalia S. ; Nguyen, Ken C. ; Rice, William J. ; Hall, David H. ; Barr, Maureen. / Cell type-specific structural plasticity of the ciliary transition zone in C. elegans. In: Biology of the Cell. 2019 ; Vol. 111, No. 4. pp. 95-107.
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Akella, JS, Silva, M, Morsci, NS, Nguyen, KC, Rice, WJ, Hall, DH & Barr, M 2019, 'Cell type-specific structural plasticity of the ciliary transition zone in C. elegans', Biology of the Cell, vol. 111, no. 4, pp. 95-107. https://doi.org/10.1111/boc.201800042

Cell type-specific structural plasticity of the ciliary transition zone in C. elegans. / Akella, Jyothi S.; Silva, Malan; Morsci, Natalia S.; Nguyen, Ken C.; Rice, William J.; Hall, David H.; Barr, Maureen.

In: Biology of the Cell, Vol. 111, No. 4, 01.04.2019, p. 95-107.

Research output: Contribution to journalArticle

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AU - Akella, Jyothi S.

AU - Silva, Malan

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Akella JS, Silva M, Morsci NS, Nguyen KC, Rice WJ, Hall DH et al. Cell type-specific structural plasticity of the ciliary transition zone in C. elegans. Biology of the Cell. 2019 Apr 1;111(4):95-107. https://doi.org/10.1111/boc.201800042