Cellular apoptosis assay of breast cancer

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Apoptosis is an energy-dependent enzymatic cell suicide process. It almost always involves the activation of caspases. In this chapter, we systemically introduce methodologies to assay caspases dependent biochemical and morphological changes in vitro breast cancer cell lines and in vivo breast cancer tissues. In addition, mitochondrial involvement is crucial to distinguish two different apoptotic pathways. Methodology to assay dissipation of mitochondrial transmembrane potential, an early event of mitochondrial involvement, is also included. Of note, since apoptotic features may not appear to the same extent depending on the context of cell types and the death-inducing insults, a common practice is to use more than one method to assess apoptosis, qualitatively and quantitatively.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-149
Number of pages11
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1406
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Annexin V/PI staining
  • Apoptosis assay
  • Breast cancer
  • Caspase activation
  • DNA laddering assay
  • In situ TUNEL staining for apoptotic cells
  • JC-1 staining

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