Cellular response to phase-separated blends of tyrosine-derived polycarbonates

Lee Ann O. Bailey, Matthew L. Becker, Jean S. Stephens, Nathan D. Gallant, Christine M. Mahoney, Newell R. Washburn, Aarti Rege, Joachim Kohn, Eric J. Amis

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Two-dimensional thin films consisting of homopolymer and discrete compositional blends of tyrosine-derived polycarbonates were prepared and characterized in an effort to elucidate the nature of different cell responses that were measured in vitro. The structurally similar blends were found to phase separate after annealing with domain sizes dependent on the overall composition. The thin polymer films were characterized with the use of atomic force microscopy (AFM), water contact angles, and time-of-flight secondary ion mass spectrometry (TOP-SIMS) and significant changes in roughness were measured following the annealing process. Genetic expression profiles of interleukin-1β and fibronectin in MC3T3-E1 osteoblasts and RAW 264.7 murine macrophages were measured at several time points, demonstrating the time and composition-dependent nature of the cell responses. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) depicted upregulation of the fibronectin gene copy numbers in each of the blends relative to the homopolymers. Moreover, the interleukin-1β expression profile was found to be compositionally dependent. The data suggest strongly that optimal composition and processing conditions can significantly affect the acute inflammatory and extracellular matrix production responses.

Original languageEnglish (US)
Pages (from-to)491-502
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume76
Issue number3
DOIs
StatePublished - Mar 1 2006

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Keywords

  • Biomaterials
  • Fibronectin
  • Interleukin-1β
  • Phase separation
  • Real-time reverse transcriptase polymerase chain reaction (RT-PCR)
  • Tyrosine-derived polycarbonate

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