Cellular uptake and cytotoxicity of chitosan-caseinophosphopeptides nanocomplexes loaded with epigallocatechin gallate

Bing Hu, Yuwen Ting, Xiaoxiong Zeng, Qingrong Huang

Research output: Contribution to journalArticlepeer-review

86 Scopus citations


Epigallocatechin gallate (EGCG) was successfully encapsulated in novel nanocomplexes assembled from bioactive peptides, caseinophosphopeptides (CPPs), and chitosan (CS), a natural cationic polymer. Their particle sizes and surface charges were determined to be in the range of 150.0 ± 4.3 nm and 32.2 ± 3.3 mV respectively. Crosslinking between the NH 3 + groups of CS with the PO - and COO - groups of CPP, as well as the hydrogen bonding were confirmed from the FTIR results. Atomic force microscopy (AFM) images showed that EGCG loaded CS-CPP nanocomplexes were spherical in shape. Maintaining the surface charge as high as +32.2 mV, crosslinking CS with peptides reduced the cytotoxicity of CS nanoparticles. In addition, cellular internalization of EGCG-loaded CS-CPP nanoparticles was confirmed from green fluorescence inside the Caco-2 cells. The process of nanoparticle uptake was dose and time dependent in the range of time and concentration studied. Furthermore, the intestinal permeability of EGCG using Caco-2 monolayer was enhanced significantly as delivered by nanoparticles, which indicated the promising elevation of EGCG bioavailability.

Original languageEnglish (US)
Pages (from-to)362-370
Number of pages9
JournalCarbohydrate Polymers
Issue number2
StatePublished - Jun 20 2012

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


  • Cellular toxicity
  • Cellular uptake
  • Chitosan-caseinophosphopeptides nanoparticles
  • Epigallocatechin gallate
  • Intestinal absorption


Dive into the research topics of 'Cellular uptake and cytotoxicity of chitosan-caseinophosphopeptides nanocomplexes loaded with epigallocatechin gallate'. Together they form a unique fingerprint.

Cite this