Cellulase production in continuous culture by Trichoderma reesei on xylose‐based media

D. W. Schafner, R. T. Toledo

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m3 occurred at pH 4.0 and a dilution rate of 0.0391 h−1 where residual substrate was 0.43 kg/m3, but no cellulase was produced. Maximum cellulase production of 0.69 FPU occurred at pH 3.5 and a dilution rate of 0.0110 h−1, where cell mass production was 2.56 kg/m3 and residual substrate was 0.15 kg/m3. Monod kinetic constants, corrected for endogenous metabolism, were 0.091 h−1, 0.469 kg/m3, 0.00923 h−1, and 0.470 kg cells/kg xylose at pH 3.5, for the maximum specific growth rate, Michaelis–Menten coefficient, endogenous metabolism coefficient, and yield coefficient, respectively. Specific growth rate fitted a maturation time model, which predicted decreasing maturation time with increasing pH.

Original languageEnglish (US)
Pages (from-to)865-869
Number of pages5
JournalBiotechnology and Bioengineering
Volume39
Issue number8
DOIs
StatePublished - Apr 5 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Keywords

  • Trichoderma reesei
  • cellulase
  • continuous culture
  • growth modeling
  • xylose

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