Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent

Ashley Grosche, Hema Sekaran, Ileana Pérez-Rodríguez, Valentin Starovoytov, Costantino Vetriani

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6T, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9˚ N. The cells were Gramstaining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1– 1.5 μm in length and 0.5 μm in width. Strain TB-6T grew between 45 and 70˚C (optimum 55– 60˚C), 0 and 35 g NaCl l‑1 (optimum 20–30 g l‑1) and pH 4.5 and 7.5 (optimum pH 5.5–6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6T occurred with H2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-D-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H2/CO2 (80: 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6T showed that this organism branched separately from the three most closely related genera, Caminibacter, Nautilia and Lebetimonas, within the family Nautiliaceae. Strain TB-6T contained several unique fatty acids in comparison with other members of the family Nautiliaceae. Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae, Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6T (= DSM 27783T = JCM 19563T).

Original languageEnglish (US)
Pages (from-to)1144-1150
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
DOIs
StatePublished - 2015

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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