Chapter 4 Screening Phage-Display Peptide Libraries for Vascular Targeted Peptides

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Scopus citations

Abstract

Depending on their physiologic location and functional state, vascular endothelial cells express surface receptors differentially. Recognition of this molecular diversity is essential for the development of targeted therapies. Random phage display peptide libraries can be selected in vitro on recombinant proteins or on intact cells. After systemic injection, selection can be performed in animals and humans in vivo for the isolation of ligands for tissue-specific receptors. For the screening of libraries on intact cells or tissues, no a priori knowledge of the targeted receptor is needed, as the recovered peptide ligands can identify their corresponding receptors. Furthermore, the isolated peptides can be used to target therapeutic chemicals, biologicals, gene therapy vectors, or diagnostic compounds to specific tissues in vivo. Protocols for the screening of phage libraries in these three settings-on proteins, on cells in vitro and in the living animal-are described in this chapter.

Original languageEnglish (US)
Title of host publicationAngiogenesis
Subtitle of host publicationIn Vivo Systems, Part B
EditorsDavid Cheresh
Pages83-106
Number of pages24
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume445
ISSN (Print)0076-6879

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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