Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis

John E. Pintar; Beth S. Schachter; Andrea B. Herman; Sally Durgerian; Dorothy T. Krieger

doi:10.1126/science.6740329

Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis

John E. Pintar, Beth S. Schachter, Andrea B. Herman, Sally Durgerian, Dorothy T. Krieger

Research output: Contribution to journal › Article › peer-review

162 Scopus citations

Abstract

Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.

Original language	English (US)
Pages (from-to)	632-634
Number of pages	3
Journal	Science
Volume	225
Issue number	4662
DOIs	https://doi.org/10.1126/science.6740329
State	Published - 1984
Externally published	Yes

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title = "Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis",

abstract = "Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.",

author = "Pintar, {John E.} and Schachter, {Beth S.} and Herman, {Andrea B.} and Sally Durgerian and Krieger, {Dorothy T.}",

year = "1984",

doi = "10.1126/science.6740329",

language = "English (US)",

volume = "225",

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journal = "Science",

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publisher = "American Association for the Advancement of Science",

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T1 - Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis

AU - Pintar, John E.

AU - Schachter, Beth S.

AU - Herman, Andrea B.

AU - Durgerian, Sally

AU - Krieger, Dorothy T.

PY - 1984

Y1 - 1984

N2 - Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.

AB - Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.

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Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis

Abstract

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