Characterization and use of a recombinant retroviral system for the analysis of drug resistant HIV

Daniel J. Medina, Peter P. Tung, Carol J. Nelson, Bhavani Sathya, Domenick Casareale, Roger K. Strair

    Research output: Contribution to journalArticlepeer-review

    8 Scopus citations

    Abstract

    A recombinant retroviral system was used for the analysis of early HIV breakthrough infection in the presence of antiviral drugs. The use of replication-defective HIV allowed a quantitative analysis of a single cycle of infection. This report characterizes this recombinant HIV system and demonstrates it's validity in comparison to standard assays. It is demonstrated that the protease inhibitor XM323 inhibits both early and late events in the HIV life-cycle, while dextran sulphate inhibits only early events. In addition, it is shown that this system can be used for detecting and quantitating drug resistant HIV. Thus, the use of this system may provide both novel information about the stage of the viral life-cycle inhibited and a preliminary assessment of the mechanism(s) responsible for breakthrough infection in the presence of antiretroviral drugs.

    Original languageEnglish (US)
    Pages (from-to)169-176
    Number of pages8
    JournalJournal of Virological Methods
    Volume71
    Issue number2
    DOIs
    StatePublished - Apr 1998

    All Science Journal Classification (ASJC) codes

    • Virology

    Keywords

    • HIV
    • Recombinant retroviral system
    • Viral RNA
    • Zidovudine

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