Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126

Luc Schuler, Sinéad M. Ní Chadhain, Yves Jouanneau, Christine Meyer, Gerben J. Zylstra, Pascal Hols, Spiros N. Agathos

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The α and β subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran degrader Terrabacter sp. strain DBF63, were identified. When overexpressed in Escherichia coli, FlnA1-FlnA2 was responsible for the angular oxidation of fluorene, 9-hydroxyfluorene, 9-fluorenone, dibenzofuran, and dibenzop-dioxin. Moreover, FlnA1-FlnA2 was able to oxidize polycyclic aromatic hydrocarbons and heteroaromatics, some of which were not oxidized by the dioxygenase from Terrabacter sp. strain DBF63. The quantification of resulting oxidation products showed that fluorene and phenanthrene were the preferred substrates of FlnA1-FlnA2.

Original languageEnglish (US)
Pages (from-to)1050-1057
Number of pages8
JournalApplied and environmental microbiology
Issue number4
StatePublished - Feb 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Ecology
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126'. Together they form a unique fingerprint.

Cite this