Characterization of bacterial cellulose nanocrystals: Effect of acid treatments and neutralization

Dılhun Keriman Arserim-Uçar, Figen Korel, Lin Shu Liu, Kit L. Yam

Research output: Contribution to journalArticlepeer-review


In this study, bacterial cellulose nanocrystals (BCNCs) were obtained from bacterial cellulose nanofibers (BCNFs) by controlled hydrolysis of sulfuric and hydrochloric acids. The influence of hydrolysis temperature and acid type with the addition of the post-treatment step was studied. The obtained BCNCs were analyzed based on the structural characterization and the properties of the nanocrystals. The BCNCs crystallinity increased, and the size of nanocrystals decreased with increasing 10 °C hydrolysis temperature for both acid hydrolysis conditions. Hydrolysis conditions with neutralization post-treatment did not alter the thermal stability of nanocrystals, and BCNCs had high thermal stability like raw BCNFs. Elemental analysis results indicated that sulfur content (S %) was very low for sulfuric acid hydrolyzed samples, and X-ray results did not show any sulfate salt peaks. Thermal stable BCNCs with high crystallinity were successfully produced to meet the process requirements in various applications, especially in the food industry.

Original languageEnglish (US)
Article number127597
JournalFood Chemistry
StatePublished - Jan 30 2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science


  • Bacterial cellulose
  • Cellulose nanocrystals
  • Gluconacetobacter xylinus
  • Hydrochloric acid
  • Sulfuric acid

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