Characterization of Blood‐Group‐H‐Active Ceramide Tetrasaccharide from Hog‐Stomach Mucosa

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Abstract

A fucose‐containing ceramide tetrasaccharide exhibiting blood‐group‐H activity was isolated from a lipid extract of hog stomach mucosa by column fractionations on DEAE‐cellulose and Florisil followed by purification on thin‐layer plates. By partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis, the structure of this glycolipid was identified as: Fucα1 →, 2Galα1 → 3Galβ1 → 4Glc1 → 1Cer; where Fuc = fucose; Gal = glucose; Gal = glucose and Cer = ceramide.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalEuropean Journal of Biochemistry
Volume43
Issue number1
DOIs
StatePublished - Mar 1974
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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