Characterization of Blood‐Group‐H‐Active Ceramide Tetrasaccharide from Hog‐Stomach Mucosa

Bronislaw L. Slomiany; Amalia Slomiany; Martin I. Horowtz

doi:10.1111/j.1432-1033.1974.tb03396.x

Characterization of Blood‐Group‐H‐Active Ceramide Tetrasaccharide from Hog‐Stomach Mucosa

Bronislaw L. Slomiany, Amalia Slomiany, Martin I. Horowtz

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A fucose‐containing ceramide tetrasaccharide exhibiting blood‐group‐H activity was isolated from a lipid extract of hog stomach mucosa by column fractionations on DEAE‐cellulose and Florisil followed by purification on thin‐layer plates. By partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis, the structure of this glycolipid was identified as: Fucα1 →, 2Galα1 → 3Galβ1 → 4Glc1 → 1Cer; where Fuc = fucose; Gal = glucose; Gal = glucose and Cer = ceramide.

Original language	English (US)
Pages (from-to)	161-165
Number of pages	5
Journal	European Journal of Biochemistry
Volume	43
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.1974.tb03396.x
State	Published - Mar 1974
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1111/j.1432-1033.1974.tb03396.x

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title = "Characterization of Blood‐Group‐H‐Active Ceramide Tetrasaccharide from Hog‐Stomach Mucosa",

abstract = "A fucose‐containing ceramide tetrasaccharide exhibiting blood‐group‐H activity was isolated from a lipid extract of hog stomach mucosa by column fractionations on DEAE‐cellulose and Florisil followed by purification on thin‐layer plates. By partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis, the structure of this glycolipid was identified as: Fucα1 →, 2Galα1 → 3Galβ1 → 4Glc1 → 1Cer; where Fuc = fucose; Gal = glucose; Gal = glucose and Cer = ceramide.",

author = "Slomiany, {Bronislaw L.} and Amalia Slomiany and Horowtz, {Martin I.}",

year = "1974",

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language = "English (US)",

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AU - Slomiany, Amalia

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PY - 1974/3

Y1 - 1974/3

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AB - A fucose‐containing ceramide tetrasaccharide exhibiting blood‐group‐H activity was isolated from a lipid extract of hog stomach mucosa by column fractionations on DEAE‐cellulose and Florisil followed by purification on thin‐layer plates. By partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis, the structure of this glycolipid was identified as: Fucα1 →, 2Galα1 → 3Galβ1 → 4Glc1 → 1Cer; where Fuc = fucose; Gal = glucose; Gal = glucose and Cer = ceramide.

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Characterization of Blood‐Group‐H‐Active Ceramide Tetrasaccharide from Hog‐Stomach Mucosa

Abstract

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