Characterization of gastric mucosal prostaglandin E2 receptor

Chi Ying Wu-Wang, Shoou Lih Wang, Amalia Slomiany, Bronislaw L. Slomiany

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


1. 1. The binding characteristics of gastric mucosal prostaglandin (PG) E2 (PGE2) receptor were investigated using mucosal cell membranes from rat stomach. The binding was found to be dependent upon PGE2 and membrane protein concentration, the time of incubation and the pH of the mixture, being highest at pH 3.0. 2. 2. Scatchard analysis of the binding data revealed a curvilinear plot with high affinity binding (Kd = 2 nM Bmax = 0.106 pmol/mg protein) and low affinity binding (Kd = 319 nM Bmax = 2.262 pmol/mg) protein sites. 3. 3. Competitive displacement study indicated that the receptor was specific for PGs of the E series, as PGF2x and 6-keto-PGF1xfailed to displace the PGE2. 4. 4. The study is the first report to provide biochemical parameters of specific PGE receptors in rat gastric mucosa.

Original languageEnglish (US)
Pages (from-to)915-919
Number of pages5
JournalInternational Journal of Biochemistry
Issue number8
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry


Dive into the research topics of 'Characterization of gastric mucosal prostaglandin E<sub>2</sub> receptor'. Together they form a unique fingerprint.

Cite this