Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues

Gaston H.M. Vondenhoff; Bart Blanchaert; Sophie Geboers; Teymur Kazakov; Kirill A. Datsenko; Barry L. Wanner; Jef Rozenski; Konstantin Severinov; Arthur Van Aerschot

doi:10.1128/JB.00172-11

Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues

Gaston H.M. Vondenhoff, Bart Blanchaert, Sophie Geboers, Teymur Kazakov, Kirill A. Datsenko, Barry L. Wanner, Jef Rozenski, Konstantin Severinov, Arthur Van Aerschot

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Microcin C (McC), a natural antibacterial compound consisting of a heptapeptide attached to a modified adenosine, is actively taken up by the YejABEF transporter, after which it is processed by cellular aminopeptidases, releasing the nonhydrolyzable aminoacyl adenylate, an inhibitor of aspartyl-tRNA synthetase. McC analogues with variable length of the peptide moiety were synthesized and evaluated in order to characterize the substrate preferences of the YejABEF transporter. It was shown that a minimal peptide chain length of 6 amino acids and the presence of an N-terminal formyl-methionyl-arginyl sequence are required for transport.

Original language	English (US)
Pages (from-to)	3618-3623
Number of pages	6
Journal	Journal of bacteriology
Volume	193
Issue number	14
DOIs	https://doi.org/10.1128/JB.00172-11
State	Published - Jul 2011

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology

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10.1128/JB.00172-11

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Vondenhoff, Gaston H.M. ; Blanchaert, Bart ; Geboers, Sophie ; Kazakov, Teymur ; Datsenko, Kirill A. ; Wanner, Barry L. ; Rozenski, Jef ; Severinov, Konstantin ; Van Aerschot, Arthur. / Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues. In: Journal of bacteriology. 2011 ; Vol. 193, No. 14. pp. 3618-3623.

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title = "Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues",

abstract = "Microcin C (McC), a natural antibacterial compound consisting of a heptapeptide attached to a modified adenosine, is actively taken up by the YejABEF transporter, after which it is processed by cellular aminopeptidases, releasing the nonhydrolyzable aminoacyl adenylate, an inhibitor of aspartyl-tRNA synthetase. McC analogues with variable length of the peptide moiety were synthesized and evaluated in order to characterize the substrate preferences of the YejABEF transporter. It was shown that a minimal peptide chain length of 6 amino acids and the presence of an N-terminal formyl-methionyl-arginyl sequence are required for transport.",

author = "Vondenhoff, {Gaston H.M.} and Bart Blanchaert and Sophie Geboers and Teymur Kazakov and Datsenko, {Kirill A.} and Wanner, {Barry L.} and Jef Rozenski and Konstantin Severinov and {Van Aerschot}, Arthur",

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Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues. / Vondenhoff, Gaston H.M.; Blanchaert, Bart; Geboers, Sophie; Kazakov, Teymur; Datsenko, Kirill A.; Wanner, Barry L.; Rozenski, Jef; Severinov, Konstantin; Van Aerschot, Arthur.

In: Journal of bacteriology, Vol. 193, No. 14, 07.2011, p. 3618-3623.

Research output: Contribution to journal › Article › peer-review

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AU - Vondenhoff, Gaston H.M.

AU - Blanchaert, Bart

AU - Geboers, Sophie

AU - Kazakov, Teymur

AU - Datsenko, Kirill A.

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AU - Severinov, Konstantin

AU - Van Aerschot, Arthur

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