Characterization of SJL T cell clones responsive to syngeneic lymphoma (RCS): RCS-specific clones are stimulated by activated B cells

R. H. DeKruyff, P. H. Brown, G. J. Thorbecke, N. M. Ponzio

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

To gain insight to the nature of the syngeneic T cell-stimulating molecules on SJL lymphoma cells (RCS), a panel of eight Ly-1+2- T cell clones that are specific for transplantable RCS has been generated. All of these clones proliferate vigorously in response to two independent RCS lines and to LPS-activated syngeneic or F1 B cell blasts, but not to unstimulated SJL spleen cells or to allogeneic B cell blasts. Only one RCS-specific clone displays a proliferative response to (SJL x BALB/c) resting spleen cells, suggesting that I-E molecules are not the source of stimulation of RCS-responsive cells. Responses of the T cell clones to both RCS and syngeneic LPS-activated B cells are inhibited by monoclonal antibodies to I-A antigens, and not by antibody to I-E antigens. These findings suggest that RCS-responsive T cells are stimulated either by syngeneic I-A(s) alone, in a form expressed on activated B cells, or by I-A(s) in combination with X, where X is a cell surface antigen present on B cells at certain stages of differentiation.

Original languageEnglish (US)
Pages (from-to)3581-3586
Number of pages6
JournalJournal of Immunology
Volume135
Issue number5
StatePublished - 1985

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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