Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases

Huiming Guo, Gopa Majmudar, Timothy C. Jensen, Chitra Biswas, Bryan P. Toole, Marion K. Gordon

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

EMMPRIN (extracellular matrix metalloproteinase inducer) also known as CD147 and basigin, is a member of the immunoglobulin family that is present on the surface of tumor cells and stimulates nearby fibroblasts to synthesize matrix metalloproteinases. Using our EMMPRIN cDNA, we have isolated a cosmid clone that contains the human EMMPRIN gene. S1 analysis with a fragment of the gene clone and primer extension of the mRNA was performed to determine the transcription start site. PCR and sequence analysis have defined the exon/intron organization of the gene and show that it is highly conserved with the mouse EMMPRIN/basigin gene. About 950 bases of the 5'-flanking region were examined for transcription factor consensus binding sites, locating three SP1 sites and two AP2 sites. The transcription start site was found to be located in a CpG island. Elements in the proximal promoter region were conserved in the human and mouse genes.

Original languageEnglish (US)
Pages (from-to)99-108
Number of pages10
JournalGene
Volume220
Issue number1-2
DOIs
StatePublished - Oct 5 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Basigin
  • CD147
  • M6
  • Tumor cell-derived collagenase stimulatory factor

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