Characterization of the phthalate permease OphD from Burkholderia cepacia ATCC 17616

Hung Kuang Chang, Gerben J. Zylstra

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The ophD gene, encoding a permease for phthalate transport, was cloned from Burkholderia cepacia ATCC 17616. Expression of the gene in Escherichia coli results in the ability to transport phthalate rapidly into the cell. Uptake inhibition experiments show that 4-hydroxyphthalate, 4- chlorophthalate, 4-methylphthalate, and cinchomeronate compete for the phthalate permease. An ophD knockout mutant of 17616 grows slightly more slowly on phthalate but is still able to take up phthalate at rates equivalent to that of the wild-type strain. This means that 17616 must have a second phthalate-inducible phthalate uptake system.

Original languageEnglish (US)
Pages (from-to)6197-6199
Number of pages3
JournalJournal of bacteriology
Volume181
Issue number19
DOIs
StatePublished - Oct 1999

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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