TY - JOUR
T1 - Characterization of the phthalate permease OphD from Burkholderia cepacia ATCC 17616
AU - Chang, Hung Kuang
AU - Zylstra, Gerben J.
PY - 1999/10
Y1 - 1999/10
N2 - The ophD gene, encoding a permease for phthalate transport, was cloned from Burkholderia cepacia ATCC 17616. Expression of the gene in Escherichia coli results in the ability to transport phthalate rapidly into the cell. Uptake inhibition experiments show that 4-hydroxyphthalate, 4- chlorophthalate, 4-methylphthalate, and cinchomeronate compete for the phthalate permease. An ophD knockout mutant of 17616 grows slightly more slowly on phthalate but is still able to take up phthalate at rates equivalent to that of the wild-type strain. This means that 17616 must have a second phthalate-inducible phthalate uptake system.
AB - The ophD gene, encoding a permease for phthalate transport, was cloned from Burkholderia cepacia ATCC 17616. Expression of the gene in Escherichia coli results in the ability to transport phthalate rapidly into the cell. Uptake inhibition experiments show that 4-hydroxyphthalate, 4- chlorophthalate, 4-methylphthalate, and cinchomeronate compete for the phthalate permease. An ophD knockout mutant of 17616 grows slightly more slowly on phthalate but is still able to take up phthalate at rates equivalent to that of the wild-type strain. This means that 17616 must have a second phthalate-inducible phthalate uptake system.
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U2 - 10.1128/jb.181.19.6197-6199.1999
DO - 10.1128/jb.181.19.6197-6199.1999
M3 - Article
C2 - 10498738
AN - SCOPUS:0032859007
SN - 0021-9193
VL - 181
SP - 6197
EP - 6199
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 19
ER -