TY - JOUR
T1 - Characterization of the rat carbonic anhydrase II gene structure
T2 - Sequence analysis of the 5' flanking region and 3' UTR
AU - McGowan, Michelle H.
AU - Neubauer, Judith A.
AU - Stolle, Catherine A.
N1 - Funding Information:
The authors gratefully acknowledge Dr. Charles Boyd and Dr. Katalin Csiszar for support in the latter stages of this work as well as Ms. Monica Varia for technical assistance. Also, special thanks to Dr. Deborah Carper for helpful discussions. This research was supported by grants from the American Heart Association (91F023) and the National Institutes of Health (HL16022 and AR40566).
PY - 1997/2/28
Y1 - 1997/2/28
N2 - The rat carbonic anhydrase II gene was characterized and found to be approximately 15.5 kb in length and to contain 7 exons and 6 introns. All intron/exon junction and branch point sequences conform to consensus sequences, and the overall rat CA II genomic structure appears to be conserved upon comparison with mouse, human, and chicken CA II genes. The putative cis-acting elements within the analyzed 1014 bp 5' flanking region include: TATA box, 4 Spl binding sites, 2 AP2 sites and putative tissue-specific β-globin-like repeat elements. A CpG island of approximately 800 bp was identified that begins about 600 bp upstream of exon 1 and extends about 200 bp into intron 1. In the 3' UTR, two polyadenylation signals (AATAAA) are present, the second of which is believed to be utilized. Northern blot analysis reveals that the 1.7 kb rat CA II mRNA is abundantly expressed in adult male brain and kidney, while negligible amounts are detected in heart and liver.
AB - The rat carbonic anhydrase II gene was characterized and found to be approximately 15.5 kb in length and to contain 7 exons and 6 introns. All intron/exon junction and branch point sequences conform to consensus sequences, and the overall rat CA II genomic structure appears to be conserved upon comparison with mouse, human, and chicken CA II genes. The putative cis-acting elements within the analyzed 1014 bp 5' flanking region include: TATA box, 4 Spl binding sites, 2 AP2 sites and putative tissue-specific β-globin-like repeat elements. A CpG island of approximately 800 bp was identified that begins about 600 bp upstream of exon 1 and extends about 200 bp into intron 1. In the 3' UTR, two polyadenylation signals (AATAAA) are present, the second of which is believed to be utilized. Northern blot analysis reveals that the 1.7 kb rat CA II mRNA is abundantly expressed in adult male brain and kidney, while negligible amounts are detected in heart and liver.
KW - 5' flanking sequence
KW - CpG island
KW - Polyadenylation signals
KW - Recombinant DNA
KW - Splice junctions
KW - Tissue expression
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U2 - 10.1016/S0378-1119(96)00700-7
DO - 10.1016/S0378-1119(96)00700-7
M3 - Article
C2 - 9074494
AN - SCOPUS:0031019080
SN - 0378-1119
VL - 186
SP - 181
EP - 188
JO - Gene
JF - Gene
IS - 2
ER -