Characterization of transcriptional regulatory domains of ankyrin repeat cofactor-1

Aihua Zhang, Chia Wei Li, J. Don Chen

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Abstract

The ankyrin repeats cofactor-1 (ANCO-1) was recently identified as a p160 coactivator-interacting protein that may inhibit transcriptional activity of nuclear receptors. Here, we have characterized the transcriptional regulatory domains of ANCO-1. Two intrinsic repression domains (RD) were identified: an N-terminal RD1 at residues 318-611 and a C-terminal RD2 at 2369-2663. ANCO-1 also contains an activation domain (AD) capable of stimulating transcription in both mammalian and yeast cells. The minimal AD was delimited to a 70-amino acid region at residues 2076-2145. Overall, full-length ANCO-1 exhibited transcriptional repressor activity, suggesting that RD domains may suppress the AD activity. We further demonstrated that ANCO-1 silencing by siRNA enhanced progesterone receptor-mediated transcription. Together, these results indicate that the transcriptional potential of ANCO-1 may be modulated by a combination of repression and activation signals.

Original languageEnglish (US)
Pages (from-to)1034-1040
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number4
DOIs
StatePublished - Jul 13 2007

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • ANCO-1
  • Ankyrin repeat cofactors
  • Histone deacetylases
  • Transcriptional activation domains
  • Transcriptional repression domains
  • p160 Coactivator

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