Chemical characterization of main bioactive constituents in Paeonia ostii seed meal and GC-MS analysis of seed oil

Xiao Tian, Sen Guo, Shanshan Zhang, Peisheng Li, Tianyi Wang, Chi Tang Ho, Min Hsiung Pan, Naisheng Bai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The seeds of tree peony (Paeonia ostii) are promulgated as emerging edible oil crops. However, biological properties of principal constituents of peony seeds were not well studied. Fifteen main constituents including suffruticosols A and B, trans-ε-viniferin, ampelopsin E, resveratrol, trans-resveratrol-4′-O-β-d-glucopyranoside, paeoniflorin, luteolin, luteolin-4′-O-β-d-glucopyranoside, apigenin, kaempferol, oleanic acid, betulinic acid, hederagenin, and caffeic acid were isolated and identified. Their cytotoxicity against human tumor cell lines (COLO205, HT-29, HepG2, AGS, and HL-60) were evaluated. Among them, trans-ε-viniferin showed the most potent cytotoxicity against HL-60 cells (IC50 5.6 μM); ampelopsin E exhibited the most obvious antiproliferative properties on COLO205 (IC50 78.1 μM) and HT-29 (IC50 4.2 μM) cells, and betulinic acid showed the strongest growth inhibitory effects on HepG2 (IC50 6.6 μM) and AGS (IC50 5.4 μM) cells. Three enzymes (tyronsinase, α-glucosidase, and acetylcholinesterase) inhibitory activities of 12 compounds were also screened. Stilbene compounds, especially suffruticosols A and B, showed a significant inhibitory activity on all three enzymes. Practical applications: The cytotoxicity of 15 main constituents from peony seeds against COLO205, HT-29, HepG2, AGS, and HL-60 cells were evaluated. Among them, trans-ε-viniferin showed the most potent cytotoxicity against HL-60 cells (IC50 5.6 μM); ampelopsin E exhibited the most obvious antiproliferative properties on COLO205 (IC50 78.1 μM) and HT-29 (IC50 4.2 μM) cells, and betulinic acid showed the strongest growth inhibitory effects on HepG2 (IC50 6.6 μM) and AGS (IC50 5.4 μM) cells. Collectively, these results suggested that Paeonia ostii seed (POS) extracts are potential candidates for anticancer agents.

Original languageEnglish (US)
Article numbere13088
JournalJournal of Food Biochemistry
Volume44
Issue number1
DOIs
StatePublished - Jan 1 2020

Fingerprint

Paeonia
seed oils
Inhibitory Concentration 50
inhibitory concentration 50
Meals
Seeds
Oils
seeds
viniferins
cytotoxicity
HL-60 Cells
Luteolin
cells
luteolin
resveratrol
Paeonia suffruticosa
Glucosidases
Apigenin
Stilbenes
glucosidases

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biophysics
  • Pharmacology
  • Cell Biology

Keywords

  • Paeonia ostii
  • cytotoxicity
  • enzyme inhibitory activities
  • seed oil fatty acids

Cite this

Tian, Xiao ; Guo, Sen ; Zhang, Shanshan ; Li, Peisheng ; Wang, Tianyi ; Ho, Chi Tang ; Pan, Min Hsiung ; Bai, Naisheng. / Chemical characterization of main bioactive constituents in Paeonia ostii seed meal and GC-MS analysis of seed oil. In: Journal of Food Biochemistry. 2020 ; Vol. 44, No. 1.
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abstract = "The seeds of tree peony (Paeonia ostii) are promulgated as emerging edible oil crops. However, biological properties of principal constituents of peony seeds were not well studied. Fifteen main constituents including suffruticosols A and B, trans-ε-viniferin, ampelopsin E, resveratrol, trans-resveratrol-4′-O-β-d-glucopyranoside, paeoniflorin, luteolin, luteolin-4′-O-β-d-glucopyranoside, apigenin, kaempferol, oleanic acid, betulinic acid, hederagenin, and caffeic acid were isolated and identified. Their cytotoxicity against human tumor cell lines (COLO205, HT-29, HepG2, AGS, and HL-60) were evaluated. Among them, trans-ε-viniferin showed the most potent cytotoxicity against HL-60 cells (IC50 5.6 μM); ampelopsin E exhibited the most obvious antiproliferative properties on COLO205 (IC50 78.1 μM) and HT-29 (IC50 4.2 μM) cells, and betulinic acid showed the strongest growth inhibitory effects on HepG2 (IC50 6.6 μM) and AGS (IC50 5.4 μM) cells. Three enzymes (tyronsinase, α-glucosidase, and acetylcholinesterase) inhibitory activities of 12 compounds were also screened. Stilbene compounds, especially suffruticosols A and B, showed a significant inhibitory activity on all three enzymes. Practical applications: The cytotoxicity of 15 main constituents from peony seeds against COLO205, HT-29, HepG2, AGS, and HL-60 cells were evaluated. Among them, trans-ε-viniferin showed the most potent cytotoxicity against HL-60 cells (IC50 5.6 μM); ampelopsin E exhibited the most obvious antiproliferative properties on COLO205 (IC50 78.1 μM) and HT-29 (IC50 4.2 μM) cells, and betulinic acid showed the strongest growth inhibitory effects on HepG2 (IC50 6.6 μM) and AGS (IC50 5.4 μM) cells. Collectively, these results suggested that Paeonia ostii seed (POS) extracts are potential candidates for anticancer agents.",
keywords = "Paeonia ostii, cytotoxicity, enzyme inhibitory activities, seed oil fatty acids",
author = "Xiao Tian and Sen Guo and Shanshan Zhang and Peisheng Li and Tianyi Wang and Ho, {Chi Tang} and Pan, {Min Hsiung} and Naisheng Bai",
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Chemical characterization of main bioactive constituents in Paeonia ostii seed meal and GC-MS analysis of seed oil. / Tian, Xiao; Guo, Sen; Zhang, Shanshan; Li, Peisheng; Wang, Tianyi; Ho, Chi Tang; Pan, Min Hsiung; Bai, Naisheng.

In: Journal of Food Biochemistry, Vol. 44, No. 1, e13088, 01.01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemical characterization of main bioactive constituents in Paeonia ostii seed meal and GC-MS analysis of seed oil

AU - Tian, Xiao

AU - Guo, Sen

AU - Zhang, Shanshan

AU - Li, Peisheng

AU - Wang, Tianyi

AU - Ho, Chi Tang

AU - Pan, Min Hsiung

AU - Bai, Naisheng

PY - 2020/1/1

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AB - The seeds of tree peony (Paeonia ostii) are promulgated as emerging edible oil crops. However, biological properties of principal constituents of peony seeds were not well studied. Fifteen main constituents including suffruticosols A and B, trans-ε-viniferin, ampelopsin E, resveratrol, trans-resveratrol-4′-O-β-d-glucopyranoside, paeoniflorin, luteolin, luteolin-4′-O-β-d-glucopyranoside, apigenin, kaempferol, oleanic acid, betulinic acid, hederagenin, and caffeic acid were isolated and identified. Their cytotoxicity against human tumor cell lines (COLO205, HT-29, HepG2, AGS, and HL-60) were evaluated. Among them, trans-ε-viniferin showed the most potent cytotoxicity against HL-60 cells (IC50 5.6 μM); ampelopsin E exhibited the most obvious antiproliferative properties on COLO205 (IC50 78.1 μM) and HT-29 (IC50 4.2 μM) cells, and betulinic acid showed the strongest growth inhibitory effects on HepG2 (IC50 6.6 μM) and AGS (IC50 5.4 μM) cells. Three enzymes (tyronsinase, α-glucosidase, and acetylcholinesterase) inhibitory activities of 12 compounds were also screened. Stilbene compounds, especially suffruticosols A and B, showed a significant inhibitory activity on all three enzymes. Practical applications: The cytotoxicity of 15 main constituents from peony seeds against COLO205, HT-29, HepG2, AGS, and HL-60 cells were evaluated. Among them, trans-ε-viniferin showed the most potent cytotoxicity against HL-60 cells (IC50 5.6 μM); ampelopsin E exhibited the most obvious antiproliferative properties on COLO205 (IC50 78.1 μM) and HT-29 (IC50 4.2 μM) cells, and betulinic acid showed the strongest growth inhibitory effects on HepG2 (IC50 6.6 μM) and AGS (IC50 5.4 μM) cells. Collectively, these results suggested that Paeonia ostii seed (POS) extracts are potential candidates for anticancer agents.

KW - Paeonia ostii

KW - cytotoxicity

KW - enzyme inhibitory activities

KW - seed oil fatty acids

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