Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination

Andrea S. Cupp, Mehmet Uzumcu, Michael K. Skinner

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to differentiate and have been shown to express neurotropin-3 (NT3), which can act on high-affinity trkC receptors expressed on migrating mesonephros cells. NT3 expression is elevated in the embryonic testis during the time of seminiferous cord formation. A trkC receptor tyrophostin inhibitor, AG879, was found to inhibit seminiferous cord formation and mesonephros cell migration. Beads containing NT3 were found to directly promote mesonephros cell migration into the gonad. Beads containing other growth factors such as epidermal growth factor (EGF) did not influence cell migration. At male sex determination the SRY gene promotes testis development and the expression of downstream sex differentiation genes such as SOX-9. Inhibition of NT3 actions caused a reduction in the expression of SOX-9. Combined observations suggest that when male sex determination is initiated, the developing Sertoli cells express NT3 as a chemotactic agent for migrating mesonephros cells, which are essential to promote embryonic testis cord formation and influence downstream male sex differentiation.

Original languageEnglish (US)
Pages (from-to)2033-2037
Number of pages5
JournalBiology of reproduction
Volume68
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Mesonephros
Testis
trkC Receptor
Cell Movement
Sex Differentiation
Sertoli Cells
Gonads
Epidermal Growth Factor
Genes
Intercellular Signaling Peptides and Proteins
neurotropin

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

Keywords

  • Developmental biology
  • Early development
  • Embryo
  • Sertoli cells
  • Testis

Cite this

@article{a628fdff34bf487b8817e8ba58bcc84f,
title = "Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination",
abstract = "The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to differentiate and have been shown to express neurotropin-3 (NT3), which can act on high-affinity trkC receptors expressed on migrating mesonephros cells. NT3 expression is elevated in the embryonic testis during the time of seminiferous cord formation. A trkC receptor tyrophostin inhibitor, AG879, was found to inhibit seminiferous cord formation and mesonephros cell migration. Beads containing NT3 were found to directly promote mesonephros cell migration into the gonad. Beads containing other growth factors such as epidermal growth factor (EGF) did not influence cell migration. At male sex determination the SRY gene promotes testis development and the expression of downstream sex differentiation genes such as SOX-9. Inhibition of NT3 actions caused a reduction in the expression of SOX-9. Combined observations suggest that when male sex determination is initiated, the developing Sertoli cells express NT3 as a chemotactic agent for migrating mesonephros cells, which are essential to promote embryonic testis cord formation and influence downstream male sex differentiation.",
keywords = "Developmental biology, Early development, Embryo, Sertoli cells, Testis",
author = "Cupp, {Andrea S.} and Mehmet Uzumcu and Skinner, {Michael K.}",
year = "2003",
month = "6",
day = "1",
doi = "10.1095/biolreprod.102.012617",
language = "English (US)",
volume = "68",
pages = "2033--2037",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "6",

}

Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination. / Cupp, Andrea S.; Uzumcu, Mehmet; Skinner, Michael K.

In: Biology of reproduction, Vol. 68, No. 6, 01.06.2003, p. 2033-2037.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination

AU - Cupp, Andrea S.

AU - Uzumcu, Mehmet

AU - Skinner, Michael K.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to differentiate and have been shown to express neurotropin-3 (NT3), which can act on high-affinity trkC receptors expressed on migrating mesonephros cells. NT3 expression is elevated in the embryonic testis during the time of seminiferous cord formation. A trkC receptor tyrophostin inhibitor, AG879, was found to inhibit seminiferous cord formation and mesonephros cell migration. Beads containing NT3 were found to directly promote mesonephros cell migration into the gonad. Beads containing other growth factors such as epidermal growth factor (EGF) did not influence cell migration. At male sex determination the SRY gene promotes testis development and the expression of downstream sex differentiation genes such as SOX-9. Inhibition of NT3 actions caused a reduction in the expression of SOX-9. Combined observations suggest that when male sex determination is initiated, the developing Sertoli cells express NT3 as a chemotactic agent for migrating mesonephros cells, which are essential to promote embryonic testis cord formation and influence downstream male sex differentiation.

AB - The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to differentiate and have been shown to express neurotropin-3 (NT3), which can act on high-affinity trkC receptors expressed on migrating mesonephros cells. NT3 expression is elevated in the embryonic testis during the time of seminiferous cord formation. A trkC receptor tyrophostin inhibitor, AG879, was found to inhibit seminiferous cord formation and mesonephros cell migration. Beads containing NT3 were found to directly promote mesonephros cell migration into the gonad. Beads containing other growth factors such as epidermal growth factor (EGF) did not influence cell migration. At male sex determination the SRY gene promotes testis development and the expression of downstream sex differentiation genes such as SOX-9. Inhibition of NT3 actions caused a reduction in the expression of SOX-9. Combined observations suggest that when male sex determination is initiated, the developing Sertoli cells express NT3 as a chemotactic agent for migrating mesonephros cells, which are essential to promote embryonic testis cord formation and influence downstream male sex differentiation.

KW - Developmental biology

KW - Early development

KW - Embryo

KW - Sertoli cells

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0037837127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037837127&partnerID=8YFLogxK

U2 - 10.1095/biolreprod.102.012617

DO - 10.1095/biolreprod.102.012617

M3 - Article

C2 - 12606390

AN - SCOPUS:0037837127

VL - 68

SP - 2033

EP - 2037

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 6

ER -