Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts

Vladimir A. Sidorov, László Menczel, Ferenc Nagy, Pál Maliga

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

Protoplasts of a light sensitive plastome mutant of Nicotiana tabacum (2 n=48) were irradiated and fused with iodoacetate-treated Nicotiana plumbaginifolia (2 n=20) protoplasts. Treated parental protoplasts were unable to divide. Metabolic complementation, however, helped the recovery of interspecific fusion products which survived and formed calli. Altogether 40 clones were investigated. N. plumbaginifolia plants were obtained in 15 clones (38%), somatic hybrids in 23 clones, and both types of regenerates were found in 2 clones. Irradiation therefore significantly increased the frequency of segregant formation with the non-irradiated N. plumbaginifolia nuclei (the frequency was 1.4% in the absence of irradiation). Regenerated plants in most cases (31 out of 34) contained chloroplasts from the irradiated parent. In 6 clones plants were obtained with both types of chloroplast. Thus, irradiated N. tabacum chloroplasts had an improved chance of dominating the heterokaryonderived cells, many of which contained N. plumbaginifolia nucleus. The system described should be generally applicable for the transfer of chloroplasts without the use of selectable genetic markers.

Original languageEnglish (US)
Pages (from-to)341-345
Number of pages5
JournalPlanta
Volume152
Issue number4
DOIs
StatePublished - Jul 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Keywords

  • Chloroplast transfer
  • Nicotiana
  • Protoplasts fusion and inactivation

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