Chloroplasts as expression platforms for plant-produced vaccines

Teodoro Cardi, Paolo Lenzi, Pal Maliga

Research output: Contribution to journalReview articlepeer-review

64 Scopus citations

Abstract

Production of recombinant subunit vaccines from genes incorporated in the plastid genome is advantageous because of the attainable expression level due to high transgene copy number and the absence of gene silencing; biocontainment as a consequence of maternal inheritance of plastids and no transgene presence in the pollen; and expression of multiple transgenes in prokaryotic-like operons. We discuss the core technology of plastid transformation in Chlamydomonas reinhardtii, a unicellular alga, and Nicotiana tabacum (tobacco), a flowering plant species, and demonstrate the utility of the technology for the production of recombinant vaccine antigens.

Original languageEnglish (US)
Pages (from-to)893-911
Number of pages19
JournalExpert Review of Vaccines
Volume9
Issue number8
DOIs
StatePublished - Aug 2010

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

Keywords

  • Chlamydomonas
  • chloroplast
  • genetic transformation
  • lettuce
  • oral delivery
  • plastid
  • production platform
  • subunit vaccine
  • tobacco
  • transplastomic plant

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