TY - JOUR
T1 - Cigarette smoking reduces human salivary eicosanoids
AU - Wu-Wang, C. Y.
AU - Wang, S. L.
AU - Lim, C.
AU - Milles, M.
AU - Slomiany, A.
AU - Slomiany, B. L.
N1 - Funding Information:
This work was supported by UMDNJ Grant 2507RRO600%-02( BRSG).
PY - 1992/10
Y1 - 1992/10
N2 - The effect of cigarette smoking on salivary eicosanoid levels was investigated in 10 smoker and 10 non-smoker volunteers. The smokers consumed an average of 20 cigarettes/day for the past 5 years or longer. The smoking status was validated by salivary cotinine level. Eicosanoids were extracted from saliva with ethanol, and the radioimmunoassay was performed to determine the concentrations of four major eicosanoids, i.e. prostaglandin E2 (PGE2), PGF2α, 6-sulphidopeptide-containing leukotrienes (LTs) and 12-hydroxyeicosatetraenoic acid (12-HETE). The levels of PGE2, PGF2α, and LTs were significantly lower in the saliva of smokers as compared to that of the non-smokers (1.74 ± 0.32 vs 2.41 ± 0.64, p=0.006; 0.36 ± 0.12 vs 0.54 ± 0.18, p=0.04; 2.24 ± 0.96 vs 4.92 ± 1.29, p=0.006; mean ± SD, ng/ml saliva). No significant differences were found in the levels of 12-HETE between the two groups. The results suggest that cigarette smoking reduces the concentrations of both the cyclooxygenase and 5-lipoxygenase products in saliva.
AB - The effect of cigarette smoking on salivary eicosanoid levels was investigated in 10 smoker and 10 non-smoker volunteers. The smokers consumed an average of 20 cigarettes/day for the past 5 years or longer. The smoking status was validated by salivary cotinine level. Eicosanoids were extracted from saliva with ethanol, and the radioimmunoassay was performed to determine the concentrations of four major eicosanoids, i.e. prostaglandin E2 (PGE2), PGF2α, 6-sulphidopeptide-containing leukotrienes (LTs) and 12-hydroxyeicosatetraenoic acid (12-HETE). The levels of PGE2, PGF2α, and LTs were significantly lower in the saliva of smokers as compared to that of the non-smokers (1.74 ± 0.32 vs 2.41 ± 0.64, p=0.006; 0.36 ± 0.12 vs 0.54 ± 0.18, p=0.04; 2.24 ± 0.96 vs 4.92 ± 1.29, p=0.006; mean ± SD, ng/ml saliva). No significant differences were found in the levels of 12-HETE between the two groups. The results suggest that cigarette smoking reduces the concentrations of both the cyclooxygenase and 5-lipoxygenase products in saliva.
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U2 - 10.1016/0952-3278(92)90144-8
DO - 10.1016/0952-3278(92)90144-8
M3 - Article
C2 - 1461919
AN - SCOPUS:0026671706
SN - 0952-3278
VL - 47
SP - 101
EP - 104
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 2
ER -