@inproceedings{b47661a022a84ec2a7cae7d5583412f0,
title = "Circumferential molecular delivery into single cells via cell-rolling mediated electroporation in microfluidic channels",
abstract = "We demonstrate a cell-rolling-mediated micro-electroporation method to circumferentially deliver biomolecules into cells. This work extends previous studies, which investigated electroporation-mediated molecular-transport mechanisms assisting cellular uptake using electrokinetic techniques to augment delivery profiles and efficiency. Using a multi-inlet geometry to control the carrier-flow profiles in the microchannel, cell flow paths and angular velocities can be controlled via hydrodynamically patterned fluid shearing. Electroporation is a fundamentally polar phenomenon where only cell surfaces perpendicular to the direction of the electric field become permeabilized. Therefore, cell rolling exposes new surfaces to the electric field to increase the permeabilized membrane area compared to non-rolling cells.",
keywords = "Cell-rolling, Electrokinetics, Electrophoresis, Electroporation, Microfluidics, Molecular-transport",
author = "Mingde Zheng and Shan, {Jerry W.} and Hao Lin and Shreiber, {David I.} and Zahn, {Jeffrey D.}",
year = "2013",
language = "English (US)",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1932--1934",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}