Clinical applications of electron microscopy in the analysis of collagenous biomaterials

A. J. Wasserman, C. J. Doillon, A. I. Glasgold, Y. P. Kato, D. Christiansen, A. Rizvi, E. Wong, J. Goldstein, Frederick Silver

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Scanning and transmission electron microscopy are of clinical value in assessing the interaction between biomaterials and ingrowing tissues. Ultrastructural information allows the clinician and biomaterials specialist to determine events occurring during wound healing and the biocompatibility of prosthetic devices. This paper reviews some of the experimental and clinical studies done in our laboratory on the use of natural and reconstituted collagen as replacements for connective tissues. Consideration is given to collagen flakes used for the treatment of dermal ulcers, a collagen fiber prosthesis used for tendon and ligament replacement, the effects of chemical preservatives on cartilage used for replacement of tissues during plastic surgery and the growth and orientation of nerve cells on reconstituted collagen fibers. Our results show that reconstituted collagen can be prepared into prosthetic devices which encourage cell attachment and orientation thereby facilitating healing of injured tissues. Furthermore chemical preservation of cartilagenous tissues kills chondrocytes resulting in eventual resorption by inflammatory cells.

Original languageEnglish (US)
Pages (from-to)1635-1646
Number of pages12
JournalScanning Microscopy
Volume2
Issue number3
StatePublished - Jan 1 1988

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collagens
Collagen
Biomaterials
Electron microscopy
electron microscopy
Tissue
prosthetic devices
Prosthetics
cells
preservatives
ulcers
connective tissue
wound healing
tendons
ligaments
cartilage
fibers
Fibers
flakes
Ligaments

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

Wasserman, A. J., Doillon, C. J., Glasgold, A. I., Kato, Y. P., Christiansen, D., Rizvi, A., ... Silver, F. (1988). Clinical applications of electron microscopy in the analysis of collagenous biomaterials. Scanning Microscopy, 2(3), 1635-1646.
Wasserman, A. J. ; Doillon, C. J. ; Glasgold, A. I. ; Kato, Y. P. ; Christiansen, D. ; Rizvi, A. ; Wong, E. ; Goldstein, J. ; Silver, Frederick. / Clinical applications of electron microscopy in the analysis of collagenous biomaterials. In: Scanning Microscopy. 1988 ; Vol. 2, No. 3. pp. 1635-1646.
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Wasserman, AJ, Doillon, CJ, Glasgold, AI, Kato, YP, Christiansen, D, Rizvi, A, Wong, E, Goldstein, J & Silver, F 1988, 'Clinical applications of electron microscopy in the analysis of collagenous biomaterials', Scanning Microscopy, vol. 2, no. 3, pp. 1635-1646.

Clinical applications of electron microscopy in the analysis of collagenous biomaterials. / Wasserman, A. J.; Doillon, C. J.; Glasgold, A. I.; Kato, Y. P.; Christiansen, D.; Rizvi, A.; Wong, E.; Goldstein, J.; Silver, Frederick.

In: Scanning Microscopy, Vol. 2, No. 3, 01.01.1988, p. 1635-1646.

Research output: Contribution to journalReview article

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AU - Doillon, C. J.

AU - Glasgold, A. I.

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AU - Christiansen, D.

AU - Rizvi, A.

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AU - Goldstein, J.

AU - Silver, Frederick

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N2 - Scanning and transmission electron microscopy are of clinical value in assessing the interaction between biomaterials and ingrowing tissues. Ultrastructural information allows the clinician and biomaterials specialist to determine events occurring during wound healing and the biocompatibility of prosthetic devices. This paper reviews some of the experimental and clinical studies done in our laboratory on the use of natural and reconstituted collagen as replacements for connective tissues. Consideration is given to collagen flakes used for the treatment of dermal ulcers, a collagen fiber prosthesis used for tendon and ligament replacement, the effects of chemical preservatives on cartilage used for replacement of tissues during plastic surgery and the growth and orientation of nerve cells on reconstituted collagen fibers. Our results show that reconstituted collagen can be prepared into prosthetic devices which encourage cell attachment and orientation thereby facilitating healing of injured tissues. Furthermore chemical preservation of cartilagenous tissues kills chondrocytes resulting in eventual resorption by inflammatory cells.

AB - Scanning and transmission electron microscopy are of clinical value in assessing the interaction between biomaterials and ingrowing tissues. Ultrastructural information allows the clinician and biomaterials specialist to determine events occurring during wound healing and the biocompatibility of prosthetic devices. This paper reviews some of the experimental and clinical studies done in our laboratory on the use of natural and reconstituted collagen as replacements for connective tissues. Consideration is given to collagen flakes used for the treatment of dermal ulcers, a collagen fiber prosthesis used for tendon and ligament replacement, the effects of chemical preservatives on cartilage used for replacement of tissues during plastic surgery and the growth and orientation of nerve cells on reconstituted collagen fibers. Our results show that reconstituted collagen can be prepared into prosthetic devices which encourage cell attachment and orientation thereby facilitating healing of injured tissues. Furthermore chemical preservation of cartilagenous tissues kills chondrocytes resulting in eventual resorption by inflammatory cells.

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Wasserman AJ, Doillon CJ, Glasgold AI, Kato YP, Christiansen D, Rizvi A et al. Clinical applications of electron microscopy in the analysis of collagenous biomaterials. Scanning Microscopy. 1988 Jan 1;2(3):1635-1646.